International Knockout Mouse Consortium

Conditional Gene Targeting

This images represent the vector design for conditional gene targeting:

Promoterless targeting:

Promoter-driven targeting:

This is based on the work in this paper:

• A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles.
  Testa G, Schaft J, van der Hoeven F, Glaser S, Anastassiadis K, Zhang Y, Hermann T, Stremmel W, Stewart AF
  Genesis. 2004;38;151-8. PMID: 15048813 DOI: 10.1002/gene.20012
  
  
  

Gene Trapping