Genetic Manipulation of ES Cells

5-18 November 2012, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

Application deadline: 6 July 2012

Summary:

This laboratory-based training course will provide a comprehensive overview and practical laboratory experience of the genetic manipulation of mouse ES cells for a broad range of applications. Recent advances in genome informatics, recombineering, transposon technology and uses of conditional gene targeting will be covered in lectures by both instructors and invited expert speakers and through interactive demonstrations. Laboratory work will focus on the culture and transfection of ES cells, design and construction of gene targeting vectors from BACs by recombineering, genotyping of gene targeting events and the practical use of transposon technology. Participants will also be trained in the informatics and practical use of public gene targeting resources being produced by the IKMC (International Knockout Mouse Consortium).

Topics

Informatics, Recombineering of Gene Targeting Vectors, ES Cell Culture, Gene Targeting in ES Cells, Transposon Technology, Modular Targeting Resources.

Click here for the course website.