International Knockout Mouse Consortium - Knowledgebase

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	IKMC (12) International Knockout Mouse Consortium		KOMP (10) The Knockout Mouse Program		EUCOMM (14) European Conditional Mouse Mutagenesis Program
	EUMMCR (6) European Mouse Mutant Cell Repository		EMMA (1) European Mouse Mutant Archive		Protocols (1) Collects all production and quality-checking lab protocols
	mirKO Micro-RNA Knockouts		Sanger MGP (29) Wellcome Trust Sanger Institute Mouse Genetics Project		Legal (1) Legal disclaimer for content on the site

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	How Can I Search the IKMC Database? The search box offers many methods of searching the IKMC database. You can specify multiple search terms separated by spaces or commas. Each of...
	06 Aug, 2010 Comments: 0

	What Is Your Quality Control (Genotyping) Strategy? Quality control screening of IKMC ES Cells can be performed in number of different ways. This page details the different tests that are...
	05 Feb, 2013 Comments: 0

	Mouse Clinic Quality Control Tests - (Mice) This page describes the end-user quality control tests we would expect to be carried out on our Mouse products. If you have data such as this...
	30 Sep, 2010 Comments: 0

	LoxP Screen This is the first step in the KOMP-CSD/EUCOMM production centre QC pipeline. Two different PCR reactions are performed to verify the 3' downstream...
	09 Sep, 2010 Comments: 0

	Which Exons Are "Floxed" by My Conditional Knockout? You can find this out yourself by following these steps: Type the name of a particular gene into the search window at www.knockoutmouse.org ....
	05 Aug, 2010 Comments: 0

Recent Articles

	What Is Your Quality Control (Genotyping) Strategy? Quality control screening of IKMC ES Cells can be performed in number of different ways. This page details the different tests that are...
	05 Feb, 2013 Comments: 0

	Screening of chimeras for germ line transmission (GLT) The LacZ short range PCR detects the presence of the LacZ reporter in the cassette in the tm1a form of the EUCOMM / KOMP allele. This can be...
	15 Dec, 2011 Comments: 0

	Neo Count (qPCR) The Neo count qPCR assay uses a hydrolysis probe assay (eg Applied Biosystems TaqMan technology) to determine the copy number of the Neo cassette in...
	15 Nov, 2011 Comments: 0

	What Are the Protocols Used in the EUCOMM and KOMP-CSD ES Cell Production Pipelines? EUCOMM Gateway protocols High Throughput Gateway (96 Well) Reactions Three Way Gateway Reaction (In tubes) EUCOMM Tissue Culture...
	02 Nov, 2011 Comments: 0

	tm1a allele conversion guide The following document outlines the genotyping strategies for detecting tm1a conversion to the b, c and d forms After treatment with a source of...
	19 Oct, 2011 Comments: 0

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