International Knockout Mouse Consortium - Knowledgebase

Quality control screening of IKMC ES Cells can be performed in number of different ways.  This page details the different tests that are carried out on our products that we report on. Production Laboratory For KOMP-CSD/EUCOMM products: LoxP Screen 3' Screen 5' Screen ...

This is the first step in the KOMP-CSD/EUCOMM production centre QC pipeline. Two different PCR reactions are performed to verify the 3' downstream loxP and gene identity: 1) Short range PCR (TRPCR) is performed between a universal primer in the 3' arm of the targeting cassette and a primer to...

This is the second step in the KOMP-CSD/EUCOMM production centre QC pipeline. Long range PCR (LRPCR) is performed between a universal primer in the 3' arm of the targeting cassette and two different genomic specific primers in the region downstream of the construct region. The...

This is the third step in the KOMP-CSD/EUCOMM production centre QC pipeline. For conditional constructs , this test (the 5' Screen) is only performed If no long range PCR (LRPCR) bands are generated in the 3' Screen. LRPCR is performed between a universal primer in the 5' arm of...

This matrix details the relationship between the production centre screens (LoxP Screen, 3' Screen, 5' Screen) and the availability of products to the distribution centres. 5' Screen loxP Screen 3' Screen LOA Screen loxP...