International Knockout Mouse Consortium - Knowledgebase

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document What Is Your Quality Control (Genotyping) Strategy?
Quality control screening of IKMC ES Cells can be performed in number of different ways.  This page details the different tests that are carried out on our products that we report on. Production Laboratory For KOMP-CSD/EUCOMM products: LoxP Screen 3' Screen 5' Screen ...
05 Feb, 2013 Comments: 0
document LR-PCR (5' and 3') Screens
The mirKO production laboratory performs long range PCR (LR-PCR) across the shorter of the two homology arms with one generic primer within the targeting cassette and a locus specific primer outside the targeting vector. Following expansion of the candidate targeted clones to...
05 Aug, 2010 Comments: 0