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What Is Your Quality Control (Genotyping) Strategy?
Quality control screening of IKMC ES Cells can be performed in number of different ways. This page details the different tests that are carried out on our products that we report on.
Production Laboratory
For KOMP-CSD/EUCOMM products:
LoxP Screen
3' Screen
5' Screen
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05 Feb, 2013
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Tests for Contamination
The EUCOMM production labs have MAP testing performed on the wild type ES cell stocks used in electroporations in order to generate ES cell clones. Each individual knockout clone is not MAP tested.
The EuMMCR unit lab tests every ES cell clone that is expanded in the lab for mycoplasma...
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05 Aug, 2010
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3' SR-PCR
The EUCOMM distribution laboratory (EuMMCR) performs one short-range PCR (SR-PCR) with a gene specific primer and a generic primer adjacent to the downstream loxP site. With this PCR we show that the downstream LoxP site is in the right distance to the targeted region.
Please Note:
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05 Aug, 2010
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