International Knockout Mouse Consortium - Knowledgebase

Yes. As part of our quality control effort, EUCOMM does produce 320 different mouse lines. EUCOMM ES cells get selected for mouse production and assigned to four of the five mouse clinics in EUCOMM. Researchers can nominate their favourite genes to be part of this set for mouse production. After...

Targeted traps (as we call them) differ from the intended conditional frameshift products in that the LoxP site within the 3' homology arm has not been confirmed and is likely to be missing (this seems to occur with some regularity with certain promoters). These are still potentially useful...

We do a mycoplasma assay on all our clones and one genotype verification using a short-range PCR method. This will take 2-3 more weeks.  The parental cell line used to generate the knockout cells has been fully MAP tested, and the actual cells you will be sent will have undergone a mycoplasma...

The EuMMCR price structure is the following: targeting vectors (intermediate or final) 200 € ~$260 one es cell line 500 € ~$650 two es cell lines for one gene 700 € ~$900 ...

ES Cells Upon user request EuMMCR thaws, expands and re-freezes several aliquots of the requested clone(s) in order to be able to provide the user with one aliquot and sustain the resource. Our controls include a PCR ensuring that the ES cell preparation is mycoplasma free and the verification or...

You can find this out yourself by following these steps: Type the name of a particular gene into the search window at www.knockoutmouse.org . For instance, searching for the gene 'Prmt1' will lead to this page: http://www.knockout mouse.org/search_results?crite ria=Prmt1 You...

EUCOMM Gateway protocols High Throughput Gateway (96 Well) Reactions Three Way Gateway Reaction (In tubes) EUCOMM Tissue Culture protocols Feeders for F6 Cells Protocol for Colony Picking Preparing DNA for Electroporation General Instructions for Single Cuvette...

Yes, the targeting cassette in EUCOMM and KOMP-CSD alleles, is flanked by FRT sites, such that it can be removed independently from the critical exon by means of Flip recombination. This results in the conditional allele (tm1c in the figure). Additional information about different strategies...

The clones labelled as "Knockout first" are expected to contain all the loxP sites as shown in the allele map. The knockout is obtained by introduction of a splice acceptor/reporter- cassette with a strong polyA site into an endogenous intron upstream of a critical exon. Should this allele be...

Summary of all IKMC allele types The wild-type is represented as a transcript with three exons. The 'floxed' exon (the exon that will be deleted for the null allele) is exon number 2. EUCOMM, KOMP-CSD Targeted mutations EUCOMM and KOMP-CSD make both promoter-driven and promoterless...