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Using Knockout-first ES cells to produce mice
Question:
I am about to generate KO animals using ES cells from KOMP knockout for a particular gene using the KO first allele promoter driven approach. If I am not wrong, I can use these cells directly to generate a KO animal without any treatment (or breeding) with recombinase (or...
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08 Feb, 2011
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What are the EUCOMM and KOMP-CSD gene targeting cassettes?
EUCOMM and KOMP-CSD Gene Targeting Cassettes
Promoter-Containi ng Targeting Cassettes
L1L2_Bact_P
The targeting cassette is flanked by L1/L2 Gateway sites for insertion into R1/R2 Gateway sites upstream of the critical exon(s) in the intermediate vector.
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31 Mar, 2011
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What are the EUCOMM and KOMP-CSD targeting vector plasmid backbones?
These are the EUCOMM and KOMP-CSD targeting vector plasmid backbones
Plasmid Backbones
This page displays descriptions of the various plasmid backbones that are used in our pipeline.
L3L4_pZero_DTA_kan
Plasmid backbone containing a L3/L4 Gateway exchange cassette including the...
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31 Mar, 2011
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How is the "critical" exon decided in CSD alleles?
A number of criteria are applied when choosing the critical exon for a KOMP-CSD or EUCOMM allele, but not every gene can have all criteria fulfilled:
1) The critical exon(s) is selected so that its floxing (removal) by application of Cre produces a frameshift in *every* protein-coding...
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27 Apr, 2011
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