The LacZ short range PCR detects the presence of the LacZ reporter in the cassette. This can be used as a rapid screen on F1 chimera progeny samples to detect likely germ line transmission. The WTSI genotyping assay is shown below
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Assay
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5' Forward Name
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5' Forward Sequence
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3' Reverse Name
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3' Reverse Sequence
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Product size
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|
LacZ
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LacZ_2_small_F
|
ATCACGACGCGCTGTATC
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LacZ_2_small_R
|
ACATCGGGCAAATAATATCG
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108bp
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