http://www.knockoutmouse.org/kb/ http://www.knockoutmouse.org/kb/entry/78/ Quality control screening of IKMC ES Cells can be performed in number of different ways. This page details the different tests that are carried out on our products that we report on. Production Laboratory For KOMP-CSD/EUCOMM... Tue, 05 Feb 2013 13:35:57 +0000 http://www.knockoutmouse.org/kb/entry/106/ The LacZ short range PCR detects the presence of the LacZ reporter in the cassette in the tm1a form of the EUCOMM / KOMP allele. This can be used as a rapid screen on F1 chimera progeny samples to detect likely germ line transmission. The WTSI... Thu, 15 Dec 2011 16:05:39 +0000 http://www.knockoutmouse.org/kb/entry/91/ The Neo count qPCR assay uses a hydrolysis probe assay (eg Applied Biosystems TaqMan technology) to determine the copy number of the Neo cassette in a sample. Homozygotes will possess two copies of Neo, heterozygotes one copy and wild type mice... Tue, 15 Nov 2011 09:33:34 +0000 http://www.knockoutmouse.org/kb/entry/23/ EUCOMM Gateway protocols High Throughput Gateway (96 Well) Reactions Three Way Gateway Reaction (In tubes) EUCOMM Tissue Culture protocols Feeders for F6 Cells Protocol for Colony Picking Preparing DNA for... Wed, 02 Nov 2011 14:40:48 +0000 http://www.knockoutmouse.org/kb/entry/105/ The following document outlines the genotyping strategies for detecting tm1a conversion to the b, c and d forms After treatment with a source of Cre recombinase to excise the critical exon and neo cassette (in promoter-driven lines), the... Wed, 19 Oct 2011 08:17:52 +0100 http://www.knockoutmouse.org/kb/entry/4/ Enter the gene symbol into the search box on the upper right and search. The resulting query summary lists one record (row) for each gene that matches your query. The Gene column displays the gene symbol, chromosome, and location. The... Mon, 19 Sep 2011 23:10:21 +0100 http://www.knockoutmouse.org/kb/entry/104/ After treatment with a source of Cre recombinase to excise the critical exon and neo cassette (in promoter-driven lines), the Tm1a allele is converted to the Tm1b form. Detection of this event and subsequent genotyping of mouse lines... Mon, 01 Aug 2011 15:44:09 +0100 http://www.knockoutmouse.org/kb/entry/103/ After treatment with a source of Flp recombinase to excise the selection cassette, the Tm1a allele is converted to the Tm1c form. Detection of this event and subsequent genotyping of mouse lines requires a modified strategy to that of... Mon, 01 Aug 2011 15:39:28 +0100 http://www.knockoutmouse.org/kb/entry/59/ The long range PCR (LR-PCR) genotyping primers may be obtained from the through the International Knockout Mouse Consortium (IKMC) website (www.knockoutmouse.org), searching for the gene target in question and following the links for details... Mon, 30 May 2011 00:28:32 +0100 http://www.knockoutmouse.org/kb/entry/102/ A number of criteria are applied when choosing the critical exon for a KOMP-CSD or EUCOMM allele, but not every gene can have all criteria fulfilled: 1) The critical exon(s) is selected so that its floxing (removal) by application of Cre... Wed, 27 Apr 2011 20:32:26 +0100 http://www.knockoutmouse.org/kb/entry/101/ These are the EUCOMM and KOMP-CSD targeting vector plasmid backbones Plasmid Backbones This page displays descriptions of the various plasmid backbones that are used in our pipeline. L3L4_pZero_DTA_kan Plasmid backbone containing... Thu, 31 Mar 2011 17:21:33 +0100 http://www.knockoutmouse.org/kb/entry/100/ EUCOMM and KOMP-CSD Gene Targeting Cassettes Promoter-Containi ng Targeting Cassettes L1L2_Bact_P The targeting cassette is flanked by L1/L2 Gateway sites for insertion into R1/R2 Gateway sites upstream of the critical exon(s) in... Thu, 31 Mar 2011 17:19:53 +0100 http://www.knockoutmouse.org/kb/entry/84/ This is a Taqman based screen in which the target primers/probe are developed within a unique wildtype region that is replaced or interrupted via homologous recombination of both vector homology arms. The data is analyzed via a multiplexed... Tue, 08 Mar 2011 22:27:42 +0000 http://www.knockoutmouse.org/kb/entry/99/ Question: I am about to generate KO animals using ES cells from KOMP knockout for a particular gene using the KO first allele promoter driven approach. If I am not wrong, I can use these cells directly to generate a KO animal without any treatment... Tue, 08 Feb 2011 16:55:32 +0000 http://www.knockoutmouse.org/kb/entry/90/ This page describes the end-user quality control tests we would expect to be carried out on our Mouse products. If you have data such as this on any of our products and you would like to share it with other IKMC resource users, please get in... Thu, 30 Sep 2010 14:52:35 +0100