Gene Details
| Marker Name(s): |
microRNA let7f-2
view this gene in MGI
|
| Marker Type: |
miRNA gene |
| Synonyms: |
Mirnlet7f2, mmu-let-7f-2 |
| Location: |
ChrX:151912346-151912428(+) |
Related Human Conditions (from OMIM) - no related Human Condition
Europhenome no data available
Mice no data available
IKMC Vectors and ES Cells
ES Cell Clones
| Cassette Type |
5 Deletion Alleles |
| Promotor Driven |
|
ES Cell Clones With Conditional Potential
No clones available.
ES Cell Clones Without Conditional Potential
| ES Cell Clone |
Targeting Vector |
ES Cell Strain |
ES Cell Line |
Allele |
Allele Type |
QC/Screening Data |
|
mirKO_ES_PuDtk_5D7
|
mirKO_TV_PuDtk_PL5A9
|
C57BL/6N-Atm1Brd/a
|
JM8A3 |
|
Deletion (Promotor Driven Cassette)
|
view data
( about )
|
Production Centre
| 5' Screen |
pass |
LoxP Screen |
- |
3' Screen |
pass |
| Loss of WT Allele (LOA) |
- |
Vector Integrity |
- |
|
Distribution Centre
| Karyotype |
-
|
Copy Number |
- |
Cells Thawed Correctly |
- |
| 5' LR-PCR |
- |
5' SR-PCR |
- |
3' SR-PCR |
- |
| LOA |
- |
LOXP |
- |
LACZ |
- |
| Chromosome 1 |
- |
Chromosome 8a |
- |
Chromosome 8b |
- |
| Chromosome 11a |
- |
Chromosome 11b |
- |
Chromosome Y |
- |
User/Mouse Clinic
| Southern Blot |
- |
Map Test |
- |
Karyotype |
- |
| TV Backbone Assay |
- |
5' LR-PCR |
- |
Loss of WT Allele (LOA) |
- |
| Neo Count (qPCR) |
- |
LacZ SR-PCR |
- |
5' Cassette Integrity |
- |
| Neo SR-PCR |
- |
Mutant Specific SR-PCR |
- |
LoxP Confirmation |
- |
| 3' LR-PCR |
- |
|
|
|
mirKO_ES_PuDtk_5D8
|
mirKO_TV_PuDtk_PL5A9
|
C57BL/6N-Atm1Brd/a
|
JM8A3 |
|
Deletion (Promotor Driven Cassette)
|
view data
( about )
|
Production Centre
| 5' Screen |
pass |
LoxP Screen |
- |
3' Screen |
pass |
| Loss of WT Allele (LOA) |
- |
Vector Integrity |
- |
|
Distribution Centre
| Karyotype |
-
|
Copy Number |
- |
Cells Thawed Correctly |
- |
| 5' LR-PCR |
- |
5' SR-PCR |
- |
3' SR-PCR |
- |
| LOA |
- |
LOXP |
- |
LACZ |
- |
| Chromosome 1 |
- |
Chromosome 8a |
- |
Chromosome 8b |
- |
| Chromosome 11a |
- |
Chromosome 11b |
- |
Chromosome Y |
- |
User/Mouse Clinic
| Southern Blot |
- |
Map Test |
- |
Karyotype |
- |
| TV Backbone Assay |
- |
5' LR-PCR |
- |
Loss of WT Allele (LOA) |
- |
| Neo Count (qPCR) |
- |
LacZ SR-PCR |
- |
5' Cassette Integrity |
- |
| Neo SR-PCR |
- |
Mutant Specific SR-PCR |
- |
LoxP Confirmation |
- |
| 3' LR-PCR |
- |
|
|
|
mirKO_ES_PuDtk_8G1
|
mirKO_TV_PuDtk_PL5A9
|
C57BL/6N-Atm1Brd/a
|
JM8A3 |
|
Deletion (Promotor Driven Cassette)
|
view data
( about )
|
Production Centre
| 5' Screen |
pass |
LoxP Screen |
- |
3' Screen |
pass |
| Loss of WT Allele (LOA) |
- |
Vector Integrity |
- |
|
Distribution Centre
| Karyotype |
-
|
Copy Number |
- |
Cells Thawed Correctly |
- |
| 5' LR-PCR |
- |
5' SR-PCR |
- |
3' SR-PCR |
- |
| LOA |
- |
LOXP |
- |
LACZ |
- |
| Chromosome 1 |
- |
Chromosome 8a |
- |
Chromosome 8b |
- |
| Chromosome 11a |
- |
Chromosome 11b |
- |
Chromosome Y |
- |
User/Mouse Clinic
| Southern Blot |
- |
Map Test |
- |
Karyotype |
- |
| TV Backbone Assay |
- |
5' LR-PCR |
- |
Loss of WT Allele (LOA) |
- |
| Neo Count (qPCR) |
- |
LacZ SR-PCR |
- |
5' Cassette Integrity |
- |
| Neo SR-PCR |
- |
Mutant Specific SR-PCR |
- |
LoxP Confirmation |
- |
| 3' LR-PCR |
- |
|
|
|
mirKO_ES_PuDtk_8G2
|
mirKO_TV_PuDtk_PL5A9
|
C57BL/6N-Atm1Brd/a
|
JM8A3 |
|
Deletion (Promotor Driven Cassette)
|
view data
( about )
|
Production Centre
| 5' Screen |
pass |
LoxP Screen |
- |
3' Screen |
pass |
| Loss of WT Allele (LOA) |
- |
Vector Integrity |
- |
|
Distribution Centre
| Karyotype |
-
|
Copy Number |
- |
Cells Thawed Correctly |
- |
| 5' LR-PCR |
- |
5' SR-PCR |
- |
3' SR-PCR |
- |
| LOA |
- |
LOXP |
- |
LACZ |
- |
| Chromosome 1 |
- |
Chromosome 8a |
- |
Chromosome 8b |
- |
| Chromosome 11a |
- |
Chromosome 11b |
- |
Chromosome Y |
- |
User/Mouse Clinic
| Southern Blot |
- |
Map Test |
- |
Karyotype |
- |
| TV Backbone Assay |
- |
5' LR-PCR |
- |
Loss of WT Allele (LOA) |
- |
| Neo Count (qPCR) |
- |
LacZ SR-PCR |
- |
5' Cassette Integrity |
- |
| Neo SR-PCR |
- |
Mutant Specific SR-PCR |
- |
LoxP Confirmation |
- |
| 3' LR-PCR |
- |
|
|
|
mirKO_ES_PuDtk_8G3
|
mirKO_TV_PuDtk_PL5A9
|
C57BL/6N-Atm1Brd/a
|
JM8A3 |
|
Deletion (Promotor Driven Cassette)
|
view data
( about )
|
Production Centre
| 5' Screen |
pass |
LoxP Screen |
- |
3' Screen |
pass |
| Loss of WT Allele (LOA) |
- |
Vector Integrity |
- |
|
Distribution Centre
| Karyotype |
-
|
Copy Number |
- |
Cells Thawed Correctly |
- |
| 5' LR-PCR |
- |
5' SR-PCR |
- |
3' SR-PCR |
- |
| LOA |
- |
LOXP |
- |
LACZ |
- |
| Chromosome 1 |
- |
Chromosome 8a |
- |
Chromosome 8b |
- |
| Chromosome 11a |
- |
Chromosome 11b |
- |
Chromosome Y |
- |
User/Mouse Clinic
| Southern Blot |
- |
Map Test |
- |
Karyotype |
- |
| TV Backbone Assay |
- |
5' LR-PCR |
- |
Loss of WT Allele (LOA) |
- |
| Neo Count (qPCR) |
- |
LacZ SR-PCR |
- |
5' Cassette Integrity |
- |
| Neo SR-PCR |
- |
Mutant Specific SR-PCR |
- |
LoxP Confirmation |
- |
| 3' LR-PCR |
- |
|
|
Targeting Vectors
| Design |
Targeting Vector |
Intermediate Vector |
Cassette |
Backbone |
Targeting Vector Genbank File |
|
211535
|
mirKO_TV_PuDtk_PL5A9
|
|
PGK_EM7_PuDtk_bGHpA |
PL611 |
view
|