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Ablim1

Datasets: Gene Details Europhenome Mice IKMC Vectors and ES Cells Other Mutants/Resources

Ablim1 was found for the following reasons:

mp_id	-	MP:0005390
MP	-	MP:0005390
mp_ontology	-	MP:0000001 \| MP:0005390 \| MP:0005508 \| MP:0003795 \| MP:0010119 \| MP:0000062
mp	-	MP:0005390

Gene Details

Marker Name(s):	actin-binding LIM protein 1 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	2210411C18Rik, 2610209L21Rik, 4833406P10Rik, 9330196J19Rik, AV079770, AW060987, AW215784, Limab1, abLIM-L, abLIM-M, abLIM-S
Location:	Chr19:57032733-57314919(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1194500	Ensembl Gene ID:	ENSMUSG00000025085	Vega Gene ID:	OTTMUSG00000016380
Entrez Gene ID:	226251	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	ABLIM1	3983
Rat	Ablim1	307989

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

Europhenome

Allele Name	Zygosity	adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary	embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	immune system	lethality-postnatal	lethality-prenatal/perinatal	life span-post-weaning/aging	limbs/digits/tail	liver/biliary system	muscle	nervous system	no phenotypic analysis	normal	other	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	skin/coat/nails	taste/olfaction	touch/vibrissae	tumorigenesis	vision/eye
Ablim1^tm1H	Hom

Legend:		No Raw Data			No Significant Annotations			Significant Annotation Present

view original data

Data provided by Europhenome

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 92118)	order	order	view details

ES Cell Clones

Cassette Type

5 Knockout-First Alleles

4 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0763_6_A12

HTGR04020_Z_4_F07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0763_6_C11

HTGR04020_Z_4_F07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0763_6_D10

HTGR04020_Z_4_F07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0763_6_F12

HTGR04020_Z_4_F07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0763_6_H11

HTGR04020_Z_4_F07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0763_6_D11

HTGR04020_Z_4_F07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0763_6_E10

HTGR04020_Z_4_F07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0763_6_F10

HTGR04020_Z_4_F07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0763_6_H10

HTGR04020_Z_4_F07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
367054	HTGR04020_Z_4_F07	PCS04020_A_D08	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
367054	HTGR04020_Z_4_F07	PCS04020_A_D08	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

Other Mutants/Resources

International Mouse Strain Resource (IMSR):	2 strains/ES cell lines
Targeted mutations in MGI:	5 mutants
Other mutations in MGI:	5 mutants

view original data

Data provided by Mouse Genome Informatics (MGI)

Acap3

Datasets: Gene Details Europhenome Mice IKMC Vectors and ES Cells Other Mutants/Resources

Acap3 was found for the following reasons:

mp_id	-	MP:0005390
MP	-	MP:0005390
mp_ontology	-	MP:0000001 \| MP:0005390 \| MP:0005508 \| MP:0003795 \| MP:0010122 \| MP:0010124
mp	-	MP:0005390

Gene Details

Marker Name(s):	ArfGAP with coiled-coil, ankyrin repeat and PH domains 3 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	Centb5, Kiaa1716-hp
Location:	Chr4:155891875-155907251(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:2153589	Ensembl Gene ID:	ENSMUSG00000029033	Vega Gene ID:	OTTMUSG00000010848
Entrez Gene ID:	140500	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	ACAP3	116983

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

Europhenome

Allele Name	Zygosity	adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary	embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	immune system	lethality-postnatal	lethality-prenatal/perinatal	life span-post-weaning/aging	limbs/digits/tail	liver/biliary system	muscle	nervous system	no phenotypic analysis	normal	other	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	skin/coat/nails	taste/olfaction	touch/vibrissae	tumorigenesis	vision/eye
Acap3^{tm1a(EUCOMM)Hmgu}	Hom

Legend:		No Raw Data			No Significant Annotations			Significant Annotation Present

view original data

Data provided by Europhenome

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

contact distributor

Acap3^{tm1a(EUCOMM)Hmgu}

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

EM:04793

ICS-HEPD0508_4_G09-1

HEPD0508_4_G09

C57BL/6NTac;C57BL/6NTac;C57BL/6N

view data
( about )

ICS

Southern Blot	na	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	pass	5' Cassette Integrity		Neo SR-PCR	na
Mutant Specific SR-PCR	pass	LoxP Confirmation	pass	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC), The European Mouse Mutant Archive (EMMA)

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 26119)	order	order	view details

ES Cell Clones

Cassette Type

3 Knockout-First Alleles

6 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0508_4_C11

PRPGS00054_A_F07

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0508_4_G09

PRPGS00054_A_F07

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	pass	Map Test	-	Karyotype	pass
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0508_4_G10

PRPGS00054_A_F07

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0508_4_B12

PRPGS00054_A_F07

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0508_4_D09

PRPGS00054_A_F07

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0508_4_D12

PRPGS00054_A_F07

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0508_4_E12

PRPGS00054_A_F07

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0508_4_F11

PRPGS00054_A_F07

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0508_4_H10

PRPGS00054_A_F07

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
44421	PRPGS00054_A_F07	PCS00054_C_F07	L1L2_Bact_P	L3L4_pD223_DTA_spec	view
44421	PRPGS00054_A_F07	PCS00054_C_F07	L1L2_Bact_P	L3L4_pD223_DTA_spec	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

Other Mutants/Resources

International Mouse Strain Resource (IMSR):	3 strains/ES cell lines
Targeted mutations in MGI:	2 mutants
Other mutations in MGI:	1 mutant

view original data

Data provided by Mouse Genome Informatics (MGI)

Acp2	Datasets: Gene Details Europhenome Mice IKMC Vectors and ES Cells Other Mutants/Resources

Acp2 was found for the following reasons:

mp_id	-	MP:0005390
MP	-	MP:0005390
mp_ontology	-	MP:0000001 \| MP:0005390 \| MP:0005508 \| MP:0003795 \| MP:0010122 \| MP:0010124
mp	-	MP:0005390

Gene Details

Marker Name(s):	acid phosphatase 2, lysosomal view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	Acp-2, LAP
Location:	Chr2:91202885-91214098(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:87882	Ensembl Gene ID:	ENSMUSG00000002103	Vega Gene ID:	OTTMUSG00000014413
Entrez Gene ID:	11432	CCDS ID:

Interpro Families

Histidine Phosphatase Superfamily, Clade 2 (IPR000560)

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	ACP2	53
Rat	Acp2	24162

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

Europhenome

Allele Name	Zygosity	adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary	embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	immune system	lethality-postnatal	lethality-prenatal/perinatal	life span-post-weaning/aging	limbs/digits/tail	liver/biliary system	muscle	nervous system	no phenotypic analysis	normal	other	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	skin/coat/nails	taste/olfaction	touch/vibrissae	tumorigenesis	vision/eye
Acp2^{tm1a(EUCOMM)Wtsi}	Hom

Legend:		No Raw Data			No Significant Annotations			Significant Annotation Present

view original data

Data provided by Europhenome

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

contact distributor

Acp2^{tm1a(EUCOMM)Wtsi}

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

EM:05128

MRC - Harwell-EPD0370_6_H05-1

EPD0370_6_H05

C57BL/6NTac;C57BL/6NTac;C57BL/6N-A^tm1Brd/a

view data
( about )

Harwell

Southern Blot	pass	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	na	5' Cassette Integrity		Neo SR-PCR	na
Mutant Specific SR-PCR	na	LoxP Confirmation	pass	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC), The European Mouse Mutant Archive (EMMA)

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 28586)	order	order	view details

ES Cell Clones

Cassette Type

18 Knockout-First Alleles

33 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0370_6_A01

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_A02

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_A04

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_D01

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_D04

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_F03

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_G02

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_H01

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_A08

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_B05

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_B06

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_B07

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_C06

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_C07

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_C08

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_E07

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_H05

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	pass	Karyotype	pass
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_H08

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0370_6_B02

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_C02

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_C03

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_D02

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_D03

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_E01

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_E03

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_E04

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_F02

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_G01

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_G04

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_H02

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_H03

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_H04

PG00080_Z_2_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_A05

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_A06

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_A07

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_B08

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_C05

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_D05

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_D06

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_D07

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_D08

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_E06

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_F05

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_F06

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_F07

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_G05

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_G06

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_G07

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_G08

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_H06

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0370_6_H07

PG00080_Z_3_D09

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
52072	PG00080_Z_3_D09	PCS00080_A_D09	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
52072	PG00080_Z_2_D09	PCS00080_A_D09	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
52072	PG00080_Z_3_D09	PCS00080_A_D09	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
52072	PG00080_Z_2_D09	PCS00080_A_D09	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

	Vectors	ES Cells
KOMP-Regeneron	Parental BAC Obtained		Projects: VG17296

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

Other Mutants/Resources

International Mouse Strain Resource (IMSR):	6 strains/ES cell lines
Targeted mutations in MGI:	3 mutants
Other mutations in MGI:	5 mutants

view original data

Data provided by Mouse Genome Informatics (MGI)

Akt2	Datasets: Gene Details Europhenome Mice IKMC Vectors and ES Cells Other Mutants/Resources

Akt2 was found for the following reasons:

mp_id	-	MP:0005390
MP	-	MP:0005390
mp_ontology	-	MP:0000001 \| MP:0005390 \| MP:0005508 \| MP:0002113 \| MP:0002896
mp	-	MP:0005390

Gene Details

Marker Name(s):	thymoma viral proto-oncogene 2 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	2410016A19Rik, AW554154, PKB, PKBbeta
Location:	Chr7:27591552-27640826(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:104874	Ensembl Gene ID:	ENSMUSG00000004056	Vega Gene ID:	OTTMUSG00000016504
Entrez Gene ID:	11652	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	AKT2	208
Rat	Akt2	25233

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

Europhenome

Allele Name	Zygosity	adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary	embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	immune system	lethality-postnatal	lethality-prenatal/perinatal	life span-post-weaning/aging	limbs/digits/tail	liver/biliary system	muscle	nervous system	no phenotypic analysis	normal	other	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	skin/coat/nails	taste/olfaction	touch/vibrissae	tumorigenesis	vision/eye
Akt2^tm1Wcs	Het
Akt2^tm1Wcs	Hom

Legend:		No Raw Data			No Significant Annotations			Significant Annotation Present

view original data

Data provided by Europhenome

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
KOMP-CSD (Project: 31126)	order	order	view details

ES Cell Clones

Cassette Type

5 Knockout-First Alleles

1 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0225_14_A02

PRPGS00056_A_H04

C57BL/6N

JM8.N4

tm1a(KOMP)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0225_14_A10

PRPGS00056_A_H04

C57BL/6N

JM8.N4

tm1a(KOMP)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	pass	Cells Thawed Correctly	-
5' LR-PCR	pass	5' SR-PCR	-	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	pass

EPD0225_14_A12

PRPGS00056_A_H04

C57BL/6N

JM8.N4

tm1a(KOMP)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0225_14_B05

PRPGS00056_A_H04

C57BL/6N

JM8.N4

tm1a(KOMP)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	pass	Cells Thawed Correctly	-
5' LR-PCR	pass	5' SR-PCR	-	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0225_14_B10

PRPGS00056_A_H04

C57BL/6N

JM8.N4

tm1a(KOMP)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	pass	Cells Thawed Correctly	-
5' LR-PCR	pass	5' SR-PCR	-	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0225_14_C04

PRPGS00056_A_H04

C57BL/6N

JM8.N4

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
40416	PRPGS00056_A_H04	PCS00056_A_H04	L1L2_Bact_P	L3L4_pD223_DTA_spec	view
40416	PRPGS00056_A_H04	PCS00056_A_H04	L1L2_Bact_P	L3L4_pD223_DTA_spec	view

	Vectors	ES Cells
NorCOMM	Design Requested		Projects: 65788

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

Other Mutants/Resources

International Mouse Strain Resource (IMSR):	27 strains/ES cell lines
International Gene Trap Consortium (IGTC):	13 gene traps
Targeted mutations in MGI:	7 mutants
Other mutations in MGI:	48 mutants

view original data

Data provided by Mouse Genome Informatics (MGI)

Aldh2

Datasets: Gene Details Europhenome Mice IKMC Vectors and ES Cells Other Mutants/Resources

Aldh2 was found for the following reasons:

mp_id	-	MP:0005390
MP	-	MP:0005390
mp_ontology	-	MP:0000001 \| MP:0005390 \| MP:0005508 \| MP:0003795 \| MP:0010122 \| MP:0010124
mp	-	MP:0005390

Gene Details

Marker Name(s):	aldehyde dehydrogenase 2, mitochondrial view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	Ahd-5, Ahd5
Location:	Chr5:121566027-121593824(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:99600	Ensembl Gene ID:	ENSMUSG00000029455	Vega Gene ID:	OTTMUSG00000023440
Entrez Gene ID:	11669	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	ALDH2	217
Rat	Aldh2	29539

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

Europhenome

Allele Name	Zygosity	adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary	embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	immune system	lethality-postnatal	lethality-prenatal/perinatal	life span-post-weaning/aging	limbs/digits/tail	liver/biliary system	muscle	nervous system	no phenotypic analysis	normal	other	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	skin/coat/nails	taste/olfaction	touch/vibrissae	tumorigenesis	vision/eye
Aldh2^{tm1a(EUCOMM)Wtsi}	Hom
Aldh2^{tm1a(EUCOMM)Wtsi}	Hom
Aldh2^{tm1a(EUCOMM)Wtsi}	Hom
Aldh2^{tm1a(EUCOMM)Wtsi}	Hom

Legend:		No Raw Data			No Significant Annotations			Significant Annotation Present

view original data

Data provided by Europhenome

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

contact distributor

Aldh2^{tm1a(EUCOMM)Wtsi}

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

EM:04096

GSF-EPD0089_4_F11-1

EPD0089_4_F11

C57BL/6NTac/Den;C57BL/6NTac/Den;C57BL/6N

view data
( about )

HMGU

Southern Blot	pass	TV Backbone Assay	na	5' LR-PCR	pass
Loss of WT Allele (LOA) qPCR	pass	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	pass
LacZ SR-PCR	na	5' Cassette Integrity		Neo SR-PCR	na
Mutant Specific SR-PCR	na	LoxP Confirmation	pass	3' LR-PCR	pass
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC), The European Mouse Mutant Archive (EMMA)

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 37155)	order	order	view details

ES Cell Clones

Cassette Type

14 Knockout-First Alleles

2 Targeted Non-Conditional Alleles

Promotorless

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0089_4_A10

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_A11

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_B11

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_C10

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_C12

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_D10

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_D12

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_E12

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_F10

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_F11

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_F12

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_G10

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_H11

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	fail
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_H12

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0089_4_B12

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0089_4_G11

PGS00037_A_B01

C57BL/6N

JM8.F6

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
42119	PGS00037_A_B01	PCS00037_A_B01	L1L2_gt0	L3L4_pZero_DTA_kan	view
42119	PGS00037_A_B01	PCS00037_A_B01	L1L2_gt0	L3L4_pZero_DTA_kan	view

	Vectors	ES Cells
KOMP-Regeneron	ES cell colonies screened / QC no positives		Projects: VG18506

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

Other Mutants/Resources

International Mouse Strain Resource (IMSR):	10 strains/ES cell lines
International Gene Trap Consortium (IGTC):	4 gene traps
Targeted mutations in MGI:	3 mutants
Other mutations in MGI:	21 mutants

view original data

Data provided by Mouse Genome Informatics (MGI)

Ankrd11

Datasets: Gene Details Europhenome Mice IKMC Vectors and ES Cells Other Mutants/Resources

Ankrd11 was found for the following reasons:

mp_id	-	MP:0005390
MP	-	MP:0005390
mp_ontology	-	MP:0000001 \| MP:0005390 \| MP:0005508 \| MP:0003795 \| MP:0010119 \| MP:0000063
mp	-	MP:0005390

Gene Details

Marker Name(s):	ankyrin repeat domain 11 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	2410104C19Rik, 3010027A04Rik, 6330578C09Rik, 9530048I21Rik, AA930108, Gm176, Yod
Location:	Chr8:122883337-122974793(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1924337	Ensembl Gene ID:	ENSMUSG00000035569	Vega Gene ID:	OTTMUSG00000037981
Entrez Gene ID:	77087	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	ANKRD11	29123
Rat	Ankrd11	365023

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

Europhenome

Allele Name	Zygosity	adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary	embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	immune system	lethality-postnatal	lethality-prenatal/perinatal	life span-post-weaning/aging	limbs/digits/tail	liver/biliary system	muscle	nervous system	no phenotypic analysis	normal	other	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	skin/coat/nails	taste/olfaction	touch/vibrissae	tumorigenesis	vision/eye
Ankrd11^Yod	Het

Legend:		No Raw Data			No Significant Annotations			Significant Annotation Present

view original data

Data provided by Europhenome

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

contact distributor

Ankrd11^{tm1a(EUCOMM)Wtsi}

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

EM:05921

G16-EPD0678_1_F03

EPD0678_1_F03

C57BL/6NTac;C57BL/6NTac;C57BL/6N-A^tm1Brd/a

view data
( about )

ICS

Southern Blot	na	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	pass	5' Cassette Integrity		Neo SR-PCR	pass
Mutant Specific SR-PCR	pass	LoxP Confirmation	pass	3' LR-PCR	na
Genotyping Comment

order

Ankrd11^Yod

EM:00380

Yoda. AYB. Sperm AGQ+

C3H.Cg-Ankrd11/H

no data reported
( about )

view original data

Data provided by The International Knockout Mouse Consortium (IKMC), The European Mouse Mutant Archive (EMMA)

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 71838)	order	order	view details

ES Cell Clones

Cassette Type

10 Knockout-First Alleles

9 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0678_1_A01

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_A02

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_A03

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_B01

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_B03

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_D02

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_E01

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_F03

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	pass	Map Test	-	Karyotype	pass
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	pass
3' LR-PCR	-

EPD0678_1_G01

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	pass	Map Test	-	Karyotype	pass
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	pass
3' LR-PCR	-

EPD0678_1_H04

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0678_1_A04

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_C03

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_C04

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_D01

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_D04

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_E02

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_E03

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_F02

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0678_1_H02

HTGR04015_Z_8_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
263049	HTGR04015_Z_8_F05	PCS04015_A_H02	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
263049	HTGR04015_Z_8_F05	PCS04015_A_H02	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

Other Mutants/Resources

International Mouse Strain Resource (IMSR):	35 strains/ES cell lines
International Gene Trap Consortium (IGTC):	19 gene traps
Targeted mutations in MGI:	2 mutants
Other mutations in MGI:	106 mutants

view original data

Data provided by Mouse Genome Informatics (MGI)

Ascc3

Datasets: Gene Details Europhenome Mice IKMC Vectors and ES Cells Other Mutants/Resources

Ascc3 was found for the following reasons:

mp_id	-	MP:0005390
MP	-	MP:0005390
mp_ontology	-	MP:0000001 \| MP:0005390 \| MP:0005371 \| MP:0005508 \| MP:0000545 \| MP:0009250 \| MP:0002109 \| MP
mp	-	MP:0005390

Gene Details

Marker Name(s):	activating signal cointegrator 1 complex subunit 3 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	ASC1p200, B630009I04Rik, BC023451, Helic1
Location:	Chr10:50592669-50851202(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1925237	Ensembl Gene ID:	ENSMUSG00000038774	Vega Gene ID:
Entrez Gene ID:	77987	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	ASCC3	10973

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

Europhenome

Allele Name	Zygosity	adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary	embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	immune system	lethality-postnatal	lethality-prenatal/perinatal	life span-post-weaning/aging	limbs/digits/tail	liver/biliary system	muscle	nervous system	no phenotypic analysis	normal	other	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	skin/coat/nails	taste/olfaction	touch/vibrissae	tumorigenesis	vision/eye
Ascc3^{tm1a(EUCOMM)Hmgu}	Het

Legend:		No Raw Data			No Significant Annotations			Significant Annotation Present

view original data

Data provided by Europhenome

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

contact distributor

Ascc3^{tm1a(EUCOMM)Hmgu}

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

EM:05034

ICS-HEPD0547_1_C09-1

HEPD0547_1_C09

C57BL/6NTac;C57BL/6NTac;C57BL/6N

view data
( about )

ICS

Southern Blot	pass	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	pass	5' Cassette Integrity		Neo SR-PCR	na
Mutant Specific SR-PCR	pass	LoxP Confirmation	pass	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC), The European Mouse Mutant Archive (EMMA)

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 45304)	order	order	view details

ES Cell Clones

Cassette Type

2 Knockout-First Alleles

1 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0547_1_C09

PRPGS00147_A_C03

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	pass	Map Test	-	Karyotype	pass
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0547_1_E12

PRPGS00147_A_C03

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0547_1_G09

PRPGS00147_A_C03

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
182158	PRPGS00147_A_C03	PCS00147_A_C03	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
182158	PRPGS00147_A_C03	PCS00147_A_C03	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

Other Mutants/Resources

International Mouse Strain Resource (IMSR):	17 strains/ES cell lines
International Gene Trap Consortium (IGTC):	13 gene traps
Targeted mutations in MGI:	2 mutants
Other mutations in MGI:	14 mutants

view original data

Data provided by Mouse Genome Informatics (MGI)

B9d1	Datasets: Gene Details Europhenome Mice IKMC Vectors and ES Cells Other Mutants/Resources

B9d1 was found for the following reasons:

mp_id	-	MP:0005390
MP	-	MP:0005390
mp_ontology	-	MP:0000001 \| MP:0005390 \| MP:0005508 \| MP:0003795 \| MP:0010122 \| MP:0010123
mp	-	MP:0005390

Gene Details

Marker Name(s):	B9 protein domain 1 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	AW045994, B9, Eppb9
Location:	Chr11:61505144-61512931(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1351471	Ensembl Gene ID:	ENSMUSG00000001039	Vega Gene ID:	OTTMUSG00000005834
Entrez Gene ID:	27078	CCDS ID:

Interpro Families

B9 Domain (IPR010796)

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	B9D1	27077
Rat	B9d1	287383

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

Europhenome

Allele Name	Zygosity	adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary	embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	immune system	lethality-postnatal	lethality-prenatal/perinatal	life span-post-weaning/aging	limbs/digits/tail	liver/biliary system	muscle	nervous system	no phenotypic analysis	normal	other	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	skin/coat/nails	taste/olfaction	touch/vibrissae	tumorigenesis	vision/eye
B9d1^{tm1a(EUCOMM)Wtsi}	Het

Legend:		No Raw Data			No Significant Annotations			Significant Annotation Present

view original data

Data provided by Europhenome

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

contact distributor

B9d1^{tm1a(EUCOMM)Wtsi}

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

EM:04160

ICS-EPD0060_2_H09-1

EPD0060_2_H09

C57BL/6NTac;C57BL/6NTac;C57BL/6N

view data
( about )

ICS

Southern Blot	na	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	pass	5' Cassette Integrity		Neo SR-PCR	na
Mutant Specific SR-PCR	pass	LoxP Confirmation	pass	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC), The European Mouse Mutant Archive (EMMA)

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 35858)	order	order	view details

ES Cell Clones

Cassette Type

9 Knockout-First Alleles

0 Targeted Non-Conditional Alleles

Promotorless

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Targeting Vector:

genbank file | image

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0060_2_A09

PGS00034_A_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0060_2_C09

PGS00034_A_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0060_2_D07

PGS00034_A_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0060_2_D08

PGS00034_A_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0060_2_D09

PGS00034_A_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0060_2_F07

PGS00034_A_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0060_2_F09

PGS00034_A_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0060_2_G08

PGS00034_A_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0060_2_H09

PGS00034_A_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	pass	Map Test	-	Karyotype	pass
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
296	PGS00034_A_H10	PCS00034_A_H10	L1L2_st0	L3L4_pZero_DTA_kan	view

	Vectors	ES Cells
KOMP-Regeneron (Project: VG19376)	order	order	view details

ES Cell Clones

Cassette Type

2 Deletion Alleles

Promotor Driven

Allele:	genbank file
Targeting Vector:	genbank file

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

No clones available.

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

19376A-C7

VG19376

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

19376A-F12

VG19376

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
	VG19376		ZEN-Ub1		view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

Other Mutants/Resources

International Mouse Strain Resource (IMSR):	3 strains/ES cell lines
Targeted mutations in MGI:	4 mutants

view original data

Data provided by Mouse Genome Informatics (MGI)

BC055324

Datasets: Gene Details Europhenome Mice IKMC Vectors and ES Cells Other Mutants/Resources

BC055324 was found for the following reasons:

mp_id	-	MP:0005390
MP	-	MP:0005390
mp_ontology	-	MP:0000001 \| MP:0005390 \| MP:0005508 \| MP:0002113 \| MP:0002896
mp	-	MP:0005390

Gene Details

Marker Name(s):	cDNA sequence BC055324 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:
Location:	Chr1:163945993-163994796(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:3590554	Ensembl Gene ID:	ENSMUSG00000041406	Vega Gene ID:	OTTMUSG00000034817
Entrez Gene ID:	381306	CCDS ID:

Interpro Families

Armadillo Type Fold (IPR016024)

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	C1orf112	55732
Rat	LOC498265	498265

view original data

Data provided by Mouse Genome Informatics (MGI)

Europhenome

Allele Name	Zygosity	adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary	embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	immune system	lethality-postnatal	lethality-prenatal/perinatal	life span-post-weaning/aging	limbs/digits/tail	liver/biliary system	muscle	nervous system	no phenotypic analysis	normal	other	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	skin/coat/nails	taste/olfaction	touch/vibrissae	tumorigenesis	vision/eye
BC055324^{tm1a(EUCOMM)Hmgu}	Het

Legend:		No Raw Data			No Significant Annotations			Significant Annotation Present

view original data

Data provided by Europhenome

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

contact distributor

BC055324^{tm1a(EUCOMM)Hmgu}

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

EM:04950

MRC - Harwell-HEPD0551_2_A04-1

HEPD0551_2_A04

C57BL/6NTac;C57BL/6NTac;C57BL/6N-A^tm1Brd/a

view data
( about )

Harwell

Southern Blot	pass	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	na	5' Cassette Integrity		Neo SR-PCR	na
Mutant Specific SR-PCR	na	LoxP Confirmation	pass	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC), The European Mouse Mutant Archive (EMMA)

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 66788)	order	order	view details

ES Cell Clones

Cassette Type

13 Knockout-First Alleles

4 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0551_2_A02

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_A03

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_A04

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	pass	Karyotype	pass
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_B03

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_B04

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_C02

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_C04

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_D03

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_D04

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_E01

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_G01

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_G02

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_H04

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0551_2_B02

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_D02

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_F03

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_2_H03

PRPGS00135_B_A08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
191738	PRPGS00135_B_A08	PCS00135_C_A08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
191738	PRPGS00135_B_A08	PCS00135_C_A08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view

	Vectors	ES Cells
KOMP-Regeneron (Project: VG17318)	order	order	view details

ES Cell Clones

Cassette Type

3 Deletion Alleles

Promotor Driven

Allele:	genbank file
Targeting Vector:	genbank file

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

No clones available.

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

17318A-B4

VG17318

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

17318C-G4

VG17318

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

17318C-H2

VG17318

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
	VG17318		ZEN-Ub1		view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

Other Mutants/Resources

International Mouse Strain Resource (IMSR):	7 strains/ES cell lines
International Gene Trap Consortium (IGTC):	3 gene traps
Targeted mutations in MGI:	3 mutants
Other mutations in MGI:	3 mutants

view original data

Data provided by Mouse Genome Informatics (MGI)

Bre	Datasets: Gene Details Europhenome Mice IKMC Vectors and ES Cells Other Mutants/Resources

Bre was found for the following reasons:

mp_id	-	MP:0005390
MP	-	MP:0005390
mp_ontology	-	MP:0000001 \| MP:0005390 \| MP:0005508 \| MP:0003795 \| MP:0010119 \| MP:0000062
mp	-	MP:0005390

Gene Details

Marker Name(s):	brain and reproductive organ-expressed protein view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	6030405P19Rik, AI429776, B830038C02Rik
Location:	Chr5:31697684-32084962(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1333875	Ensembl Gene ID:	ENSMUSG00000052139	Vega Gene ID:	OTTMUSG00000023678
Entrez Gene ID:	107976	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	BRE	9577
Rat	Bre	362704

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

Europhenome

Allele Name	Zygosity	adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary	embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	immune system	lethality-postnatal	lethality-prenatal/perinatal	life span-post-weaning/aging	limbs/digits/tail	liver/biliary system	muscle	nervous system	no phenotypic analysis	normal	other	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	skin/coat/nails	taste/olfaction	touch/vibrissae	tumorigenesis	vision/eye
Bre^{tm1a(EUCOMM)Wtsi}	Hom

Legend:		No Raw Data			No Significant Annotations			Significant Annotation Present

view original data

Data provided by Europhenome

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

contact distributor

Bre^{tm1a(EUCOMM)Wtsi}

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

EM:04264

MRC - Harwell-EPD0065_4_D03-1

EPD0065_4_D03

C57BL/6Dnk;C57BL/6Dnk;C57BL/6N

view data
( about )

Harwell

Southern Blot	pass	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	na	5' Cassette Integrity		Neo SR-PCR	na
Mutant Specific SR-PCR	na	LoxP Confirmation	pass	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC), The European Mouse Mutant Archive (EMMA)

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 37173)	order	order	view details

ES Cell Clones

Cassette Type

17 Knockout-First Alleles

0 Targeted Non-Conditional Alleles

Promotorless

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Targeting Vector:

genbank file | image

view all available ES cell clones

ES Cells with Knockout First mutation (Reporter-tagged insertion with conditional potential)

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0065_4_A01

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	pass

EPD0065_4_A02

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_A03

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_B02

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	pass

EPD0065_4_B03

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_C01

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_C02

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_C03

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_D03

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	pass	Map Test	-	Karyotype	pass
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_E01

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_E02

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_E03

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_F02

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_F03

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_G01

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_G02

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0065_4_H01

PGS00037_A_F08

C57BL/6N

JM8.F6

tm1a(EUCOMM)Wtsi

Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
42215	PGS00037_A_F08	PCS00037_A_F08	L1L2_gt2	L3L4_pZero_DTA_kan	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

Other Mutants/Resources

International Mouse Strain Resource (IMSR):	14 strains/ES cell lines
International Gene Trap Consortium (IGTC):	7 gene traps
Targeted mutations in MGI:	1 mutant
Other mutations in MGI:	16 mutants

view original data

Data provided by Mouse Genome Informatics (MGI)

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