IKMC Project Report - EUCOMM (ID: 113884)

Mab21l1 MGI:1333773 ENSMUSG00000056947
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - No QC Positives

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout-First - Reporter Tagged Insertion'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000075422 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MIAAQAKLVYHLNKYYNE

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000517905 Mab-21_dom (17/359 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000517905 Mab-21_dom (342/359 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR04028_Z_4_D04 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_E03 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR04028_Z_4_H06 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR04028_Z_4_B09 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_E02 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_A12 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_E11 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_A02 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_E12 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_G10 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR04028_Z_4_B10 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_A03 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_A11 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_E04 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_A04 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 400662 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00262_Z_7_C10 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
400662 Knockout-First - Reporter Tagged Insertion PCS04028_A_D03