IKMC Project Report - EUCOMM (ID: 114373)

U2af1l4 MGI:2678374 ENSMUSG00000078765 OTTMUSG00000036722
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 38 wild type transcripts, of which 9 are protein-coding. Following removal of the floxed region, 9 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000043273 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQ

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000889026 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000999439 Znf_CCCH (24/24 aa) CDS CDS
ENSMUSE00000985675 CDS Deleted
ENSMUSE00001011584 CDS Deleted
ENSMUSE00001079430 RRM_dom (28/53 aa) CDS Deleted
ENSMUSE00000990883 Znf_CCCH (11/25 aa) | RRM_dom (25/53 aa) CDS Deleted
ENSMUSE00001011447 Znf_CCCH (15/25 aa) CDS Deleted
ENSMUSE00000902385 CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000163464 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQ

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000902084 CDS CDS
ENSMUSE00000999439 Znf_CCCH (24/24 aa) CDS CDS
ENSMUSE00000985675 CDS Deleted
ENSMUSE00001011584 CDS Deleted
ENSMUSE00001079430 RRM_dom (28/53 aa) CDS Deleted
ENSMUSE00000911004 RRM_dom (25/53 aa) CDS Deleted
ENSMUSE00001011447 CDS Deleted
ENSMUSE00001063638 CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000166510 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQ

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000886186 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000999439 Znf_CCCH (24/24 aa) CDS CDS
ENSMUSE00000985675 CDS Deleted
ENSMUSE00000904725 CDS Deleted
ENSMUSE00001079430 RRM_dom (28/53 aa) CDS Deleted
ENSMUSE00000990883 Znf_CCCH (11/25 aa) | RRM_dom (25/53 aa) CDS Deleted
ENSMUSE00001011447 Znf_CCCH (15/25 aa) CDS Deleted
ENSMUSE00000888564 CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000163330 protein_coding Design floxes first exon in transcript ENSMUST00000163330
ENSMUST00000163654 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQ

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000884811 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000999439 Znf_CCCH (24/24 aa) CDS CDS
ENSMUSE00000985675 CDS Deleted
ENSMUSE00001011584 CDS Deleted
ENSMUSE00001079430 RRM_dom (28/53 aa) CDS Deleted
ENSMUSE00000990883 RRM_dom (25/53 aa) CDS Deleted
ENSMUSE00000514673 CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000171850 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQ

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000902084 CDS CDS
ENSMUSE00000999439 Znf_CCCH (24/24 aa) CDS CDS
ENSMUSE00000985675 CDS Deleted
ENSMUSE00001011584 CDS Deleted
ENSMUSE00000990883 Znf_CCCH (11/25 aa) CDS Deleted
ENSMUSE00001011447 Znf_CCCH (15/25 aa) CDS Deleted
ENSMUSE00001063638 CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000166257 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQ

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000902084 CDS CDS
ENSMUSE00000999439 Znf_CCCH (24/24 aa) | RRM_dom (1/59 aa) CDS CDS
ENSMUSE00001079430 RRM_dom (33/59 aa) CDS Deleted
ENSMUSE00000990883 Znf_CCCH (11/25 aa) | RRM_dom (25/59 aa) CDS Deleted
ENSMUSE00001011447 Znf_CCCH (15/25 aa) CDS Deleted
ENSMUSE00001063638 CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000168931 protein_coding No analysis available: incomplete transcript
ENSMUST00000163482 protein_coding No protein product
ENSMUST00000167042 nonsense_mediated_decay No protein product (NMD) view

Predicted translation: 

MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQITSSRKTAGSMTSAIL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000882847 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000999439 Znf_CCCH (24/24 aa) CDS CDS
ENSMUSE00000985675 CDS Deleted
ENSMUSE00001011584 CDS Deleted
ENSMUSE00001079430 RRM_dom (28/53 aa) CDS Deleted
ENSMUSE00000990883 Znf_CCCH (11/25 aa) | RRM_dom (25/53 aa) CDS Deleted
ENSMUSE00001011447 Znf_CCCH (15/25 aa) CDS Deleted
ENSMUSE00001027706 CDS + stop codon + UTR Deleted
ENSMUSE00000896866 UTR CDS + stop codon + UTR
ENSMUSE00000675808 UTR UTR
Legend: in frame deleted frameshifted
ENSMUST00000171912 nonsense_mediated_decay No protein product (NMD) view

Predicted translation: 

MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQITSSRKTAGSMTSAIL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000902134 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000999439 Znf_CCCH (24/24 aa) CDS CDS
ENSMUSE00000985675 CDS Deleted
ENSMUSE00001011584 CDS Deleted
ENSMUSE00001079430 RRM_dom (28/53 aa) CDS Deleted
ENSMUSE00000990883 Znf_CCCH (11/25 aa) | RRM_dom (25/53 aa) CDS Deleted
ENSMUSE00001011447 Znf_CCCH (15/25 aa) CDS Deleted
ENSMUSE00001027706 CDS + stop codon + UTR Deleted
ENSMUSE00001075411 UTR CDS + stop codon + UTR
ENSMUSE00000897493 UTR UTR
ENSMUSE00000889867 UTR UTR
Legend: in frame deleted frameshifted
ENSMUST00000108161 nonsense_mediated_decay No protein product (NMD) view

Predicted translation: 

MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQITSSRKTAGSMTSAIL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000414188 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000999439 Znf_CCCH (24/24 aa) CDS CDS
ENSMUSE00000985675 CDS Deleted
ENSMUSE00001011584 CDS Deleted
ENSMUSE00000983964 CDS + stop codon + UTR Deleted
ENSMUSE00001048379 UTR Deleted
ENSMUSE00001017462 UTR Deleted
ENSMUSE00001067985 UTR Deleted
ENSMUSE00001075411 UTR CDS + stop codon + UTR
ENSMUSE00001024119 UTR UTR
ENSMUSE00000874567 UTR UTR
Legend: in frame deleted frameshifted
ENSMUST00000168333 nonsense_mediated_decay Residual N-terminal, unknown C-terminal view

Predicted translation: 

MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQ

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000902084 CDS CDS
ENSMUSE00000999439 Znf_CCCH (24/24 aa) CDS CDS
ENSMUSE00000985675 CDS Deleted
ENSMUSE00001011584 CDS Deleted
ENSMUSE00000882290 CDS Deleted
ENSMUSE00001069956 CDS + stop codon + UTR Deleted
ENSMUSE00001017462 UTR Deleted
ENSMUSE00000999211 UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000168229 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000168555 nonsense_mediated_decay Residual N-terminal, unknown C-terminal view

Predicted translation: 

MAEYLASIFGTEKDKLEPAGTGTGAPDFTTNRLSA

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000907578 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001030659 CDS CDS + stop codon + UTR
ENSMUSE00001003231 CDS + stop codon + UTR Deleted
ENSMUSE00000905830 UTR Deleted
ENSMUSE00001017462 UTR Deleted
ENSMUSE00000895999 UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000167501 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000163276 nonsense_mediated_decay Residual N-terminal, unknown C-terminal view

Predicted translation: 

MAEYLASIFGTEKDKLEPAGTGTGAPDFTTNRLSA

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000887962 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001030659 CDS CDS + stop codon + UTR
ENSMUSE00001003231 CDS + stop codon + UTR Deleted
ENSMUSE00001046528 UTR Deleted
ENSMUSE00001057028 UTR Deleted
ENSMUSE00000877995 UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000163848 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000165722 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000164365 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000165773 retained_intron No analysis available: incomplete transcript
ENSMUST00000167361 processed_transcript No analysis available: incomplete transcript
ENSMUST00000166960 processed_transcript No analysis available: incomplete transcript
ENSMUST00000169283 retained_intron No analysis available: incomplete transcript
ENSMUST00000168222 retained_intron No analysis available: incomplete transcript
ENSMUST00000169911 retained_intron No analysis available: incomplete transcript
ENSMUST00000164438 retained_intron No analysis available: incomplete transcript
ENSMUST00000169071 retained_intron No analysis available: incomplete transcript
ENSMUST00000165009 retained_intron No analysis available: incomplete transcript
ENSMUST00000165524 processed_transcript No analysis available: incomplete transcript
ENSMUST00000169962 retained_intron No analysis available: incomplete transcript
ENSMUST00000167024 retained_intron No analysis available: incomplete transcript
ENSMUST00000165543 retained_intron No analysis available: incomplete transcript
ENSMUST00000170453 retained_intron No analysis available: incomplete transcript
ENSMUST00000167202 processed_transcript No analysis available: incomplete transcript
ENSMUST00000163994 retained_intron No analysis available: incomplete transcript
ENSMUST00000166864 processed_transcript No analysis available: incomplete transcript
ENSMUST00000171364 processed_transcript No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_Y_3_E03 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_Y_3_C11 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_Y_3_G04 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_Y_3_B07 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_3_G07 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_W_2_G01 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_Y_3_D11 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_3_G08 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_Y_3_G07 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_3_D01 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_Y_3_D01 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_W_3_F04 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_Y_3_G08 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_W_3_E12 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_Y_3_H01 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_3_H01 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_3_D11 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_3_C11 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_3_G01 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_3_B07 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_W_3_H07 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_3_G04 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_3_E03 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_Y_3_G01 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446638 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_W_3_H09 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
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Intermediate Vectors no data available