IKMC Project Report - KOMP-CSD (ID: 115794)

Wapal MGI:2675859 ENSMUSG00000041408 OTTMUSG00000024463
Pre-pipeline Designs Vectors ES Cells Mice
Vector Construction in Progress

Mice no data available

Mutagenesis Predictions

This gene has 7 wild type transcripts, of which 5 are protein-coding. Following removal of the floxed region, 5 transcripts are predicted to produce a truncated protein product of which 3 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000048263 protein_coding No protein product (NMD) view

Predicted translation: 

MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEEDTGDPFGFDSDDE
SLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVFEANSKCSHVVGEDSFASDRCLLVEDTLIGKEKSISRIPEDNANKSSC
TKLLTSDKVENFSEEHEKNSHHFHKNAEDSTKKPNAETAVASEYKADETKETNDTWNSQSGKRTESPSESCPVKGSVRTG
LYEWDNDFEDIRSEDCILSLDNESLLEMKDEDLKNRIGGLENLNETFEEDIIQSVLRPSNCRTYCRANKARSSQGASNFD
KLMDGTSQSLAKANSESSKDGLNQAKKGSASCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADL
GEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDDDDCQV
ERKKDKKRTKTAPSPSQQPPPESSDNSQDSQSSTNNAENLDFTEDLPGVPESVKKPISKQGDKSKENTRKIFSGPKRSPT
KAVYNARHWNHPDSEELPGPPIAKPQRVTCYQLSY*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000412292 UTR UTR
ENSMUSE00000617960 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000330579 CDS CDS
ENSMUSE00001067525 CDS CDS
ENSMUSE00001017201 CDS CDS
ENSMUSE00001046994 CDS Deleted
ENSMUSE00000993653 WAPL_prot (41/365 aa) CDS Deleted
ENSMUSE00001030262 WAPL_prot (38/365 aa) CDS CDS + stop codon + UTR
ENSMUSE00001029385 WAPL_prot (76/365 aa) CDS
ENSMUSE00000976464 WAPL_prot (38/365 aa) CDS
ENSMUSE00001013132 WAPL_prot (34/365 aa) CDS
ENSMUSE00000971193 WAPL_prot (26/365 aa) CDS
ENSMUSE00000983148 WAPL_prot (60/365 aa) CDS
ENSMUSE00001044696 WAPL_prot (39/365 aa) CDS
ENSMUSE00001094871 WAPL_prot (17/365 aa) CDS
ENSMUSE00001051443 CDS
ENSMUSE00001040974 CDS
ENSMUSE00001032361 CDS
ENSMUSE00000836931 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000169910 protein_coding No protein product (NMD) view

Predicted translation: 

MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEEDTGDPFGFDSDDE
SLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVFEANSKCSHVVGEDSFASDRCLLVEDTLIGKEKSISRIPEDNANKSSC
TKLLTSDKVENFSEEHEKNSHHFHKNAEDSTKKPNAETAVASEYKADETKETNDTWNSQSGKRTESPSESCPVKGSVRTG
LYEWDNDFEDIRSEDCILSLDNESLLEMKDEDLKNRIGGLENLNETFEEDIIQSVLRPSNCRTYCRANKARSSQGASNFD
KLMDGTSQSLAKANSESSKDGLNQAKKGSASCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADL
GEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDDDDCQV
ERKKDKKRTKTAPSPSQQPPPESSDNSQDSQSSTNNAENLDFTEDLPGVPESVKKPISKQGDKSKENTRKIFSGPKRSPT
KAVYNARHWNHPDSEELPGPPIAKPQRVTCYQLSY*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000894811 UTR UTR
ENSMUSE00000617960 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000330579 CDS CDS
ENSMUSE00001067525 CDS CDS
ENSMUSE00001017201 CDS CDS
ENSMUSE00001046994 CDS Deleted
ENSMUSE00000993653 WAPL_prot (41/365 aa) CDS Deleted
ENSMUSE00001030262 WAPL_prot (38/365 aa) CDS CDS + stop codon + UTR
ENSMUSE00001029385 WAPL_prot (76/365 aa) CDS
ENSMUSE00000976464 WAPL_prot (38/365 aa) CDS
ENSMUSE00001013132 WAPL_prot (34/365 aa) CDS
ENSMUSE00000971193 WAPL_prot (26/365 aa) CDS
ENSMUSE00000983148 WAPL_prot (60/365 aa) CDS
ENSMUSE00001044696 WAPL_prot (39/365 aa) CDS
ENSMUSE00001094871 WAPL_prot (17/365 aa) CDS
ENSMUSE00001051443 CDS
ENSMUSE00001040974 CDS
ENSMUSE00001032361 CDS
ENSMUSE00000913190 CDS + stop codon + UTR
ENSMUSE00000886646 UTR UTR
Legend: in frame deleted frameshifted
ENSMUST00000090027 protein_coding No protein product (NMD) view

Predicted translation: 

MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEEDTGDPFGFDSDDE
SLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVFEANSKCSHVVGEDSFASDRCLLVEDTLIGKEKSISRIPEDNANKSSC
TKLLTSDKVENFSEEHEKNSHHFHKNAEDSTKKPNAETAVASEYKADETKETNDTWNSQSGKRTESPSESCPVKGSVRTG
LYEWDNDFEDIRSEDCILSLDNESLLEMKDEDLKNRIGGLENLNETFEEDIIQSVLRPSNCRTYCRANKARSSQGASNFD
KLMDGTSQSLAKANSESSKDGLNQAKKGSASCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADL
GEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDDDDCQV
ERKKDKKRTKTAPSPSQQPPPESSDNSQDSQSSTNNAEDLPGVPESVKKPISKQGDKSKENTRKIFSGPKRSPTKAVYNA
RHWNHPDSEELPGPPIAKPQRVTCYQLSY*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000689495 UTR UTR
ENSMUSE00001091078 UTR UTR
ENSMUSE00000617960 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000330579 CDS CDS
ENSMUSE00000689494 CDS CDS
ENSMUSE00001017201 CDS CDS
ENSMUSE00001046994 CDS Deleted
ENSMUSE00000993653 WAPL_prot (41/365 aa) CDS Deleted
ENSMUSE00001030262 WAPL_prot (38/365 aa) CDS CDS + stop codon + UTR
ENSMUSE00001029385 WAPL_prot (76/365 aa) CDS
ENSMUSE00000976464 WAPL_prot (38/365 aa) CDS
ENSMUSE00001013132 WAPL_prot (34/365 aa) CDS
ENSMUSE00000971193 WAPL_prot (26/365 aa) CDS
ENSMUSE00000983148 WAPL_prot (60/365 aa) CDS
ENSMUSE00001044696 WAPL_prot (39/365 aa) CDS
ENSMUSE00001094871 WAPL_prot (17/365 aa) CDS
ENSMUSE00001051443 CDS
ENSMUSE00001040974 CDS
ENSMUSE00001032361 CDS
ENSMUSE00000561561 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000151285 protein_coding Target region does not overlap transcript
ENSMUST00000174559 protein_coding Target region does not overlap transcript
ENSMUST00000111895 processed_transcript No analysis available: incomplete transcript
ENSMUST00000172658 processed_transcript No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available