IKMC Project Report - EUCOMM (ID: 115887)

Inf2 MGI:1917685 ENSMUSG00000037679
Pre-pipeline Designs Vectors ES Cells Mice
Vector Unsuccessful - Project Terminated

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000101029 protein_coding No protein product (NMD) view

Predicted translation:

MSVKEGAQRKWAALKEKLGPQDSDPTEANLESAEPELCIRLLQMPSVVNYSGLRKRLESSDGGWMVQFLEQSGLDLLLEA
LARLSGRGVARISDALLQLTCISCVRAVMNSQQGIEYILSNQGYVRQLSQALDTSNVMVKKQVFELLAALCIYSPEGHAL
TLDALDHYKMVCSQQYRFSVIMSELSDSDNVPYVVTLLSVINAIILGPEDLRSRAQLRSEFIGLQLLDILTRLRAQLHVG
HTGQPLHSRSGARLLQH*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000681497 UTR UTR
ENSMUSE00000878625 Drf_GTPase-bd (109/131 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000308619 Drf_FH3 (13/187 aa) | Drf_GTPase-bd (23/131 aa) CDS CDS
ENSMUSE00000308614 Drf_FH3 (54/187 aa) CDS CDS
ENSMUSE00000681495 Drf_FH3 (12/187 aa) CDS CDS
ENSMUSE00000681494 Drf_FH3 (48/187 aa) CDS Deleted
ENSMUSE00000681493 Drf_FH3 (48/187 aa) CDS Deleted
ENSMUSE00000652073 FH2_actin-bd (23/363 aa) | Drf_FH3 (15/187 aa) CDS Deleted
ENSMUSE00000356745 FH2_actin-bd (49/363 aa) CDS CDS + stop codon + UTR
ENSMUSE00000369218 FH2_actin-bd (21/363 aa) CDS
ENSMUSE00000412233 FH2_actin-bd (35/363 aa) CDS
ENSMUSE00000371824 FH2_actin-bd (29/363 aa) CDS
ENSMUSE00000394107 FH2_actin-bd (35/363 aa) CDS
ENSMUSE00000307848 FH2_actin-bd (24/363 aa) CDS
ENSMUSE00000307840 FH2_actin-bd (36/363 aa) CDS
ENSMUSE00000307832 FH2_actin-bd (24/363 aa) CDS
ENSMUSE00000307824 FH2_actin-bd (41/363 aa) CDS
ENSMUSE00000307812 FH2_actin-bd (51/363 aa) CDS
ENSMUSE00000307798 CDS
ENSMUSE00000308488 WH2_dom (16/16 aa) CDS
ENSMUSE00000308484 CDS
ENSMUSE00000652071 CDS + stop codon + UTR
ENSMUSE00001014197 UTR UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available