IKMC Project Report - KOMP-CSD (ID: 116633)

Hsd11b1 MGI:103562 ENSMUSG00000016194 OTTMUSG00000030692
Pre-pipeline Designs Vectors ES Cells Mice
Vector Construction in Progress

Mice no data available

Mutagenesis Predictions

This gene has 6 wild type transcripts, of which 6 are protein-coding. Following removal of the floxed region, 6 transcripts are predicted to produce a truncated protein product of which 3 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000016338 protein_coding No protein product (NMD) view

Predicted translation:

MAVMKNYLLPILVLFLAYYYYSTNEEFRPEMLQGKKVIVTGASKGIGREMAYHLSKMGAHVVLTARSEEGLQKGK*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000853568 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000134156 DH_sc/Rdtase_SDR (37/166 aa) | PKS_KR (36/164 aa) CDS CDS
ENSMUSE00000134155 DH_sc/Rdtase_SDR (38/166 aa) | PKS_KR (38/164 aa) CDS Deleted
ENSMUSE00000284550 DH_sc/Rdtase_SDR (63/166 aa) | PKS_KR (63/164 aa) CDS Deleted
ENSMUSE00000284544 DH_sc/Rdtase_SDR (30/166 aa) | PKS_KR (29/164 aa) CDS CDS + stop codon + UTR
ENSMUSE00000861704 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000161737 protein_coding No protein product (NMD) view

Predicted translation:

MAVMKNYLLPILVLFLAYYYYSTNEEFRPEMLQGKKVIVTGASKGIGREMAYHLSKMGAHVVLTARSEEGLQKGK*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000854733 UTR UTR
ENSMUSE00001026074 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000134156 DH_sc/Rdtase_SDR (37/166 aa) | PKS_KR (36/164 aa) CDS CDS
ENSMUSE00000134155 DH_sc/Rdtase_SDR (38/166 aa) | PKS_KR (38/164 aa) CDS Deleted
ENSMUSE00000284550 DH_sc/Rdtase_SDR (63/166 aa) | PKS_KR (63/164 aa) CDS Deleted
ENSMUSE00000284544 DH_sc/Rdtase_SDR (30/166 aa) | PKS_KR (29/164 aa) CDS CDS + stop codon + UTR
ENSMUSE00000859515 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000160929 protein_coding No protein product (NMD) view

Predicted translation:

MLQGKKVIVTGASKGIGREMAYHLSKMGAHVVLTARSEEGLQKGK*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000861150 DH_sc/Rdtase_SDR (37/166 aa) | PKS_KR (36/163 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000134155 DH_sc/Rdtase_SDR (38/166 aa) | PKS_KR (38/163 aa) CDS Deleted
ENSMUSE00000284550 DH_sc/Rdtase_SDR (63/166 aa) | PKS_KR (63/163 aa) CDS Deleted
ENSMUSE00000284544 DH_sc/Rdtase_SDR (30/166 aa) | PKS_KR (28/163 aa) CDS CDS + stop codon + UTR
ENSMUSE00000864638 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000159644 protein_coding No analysis available: incomplete transcript
ENSMUST00000162842 protein_coding No analysis available: incomplete transcript
ENSMUST00000161406 protein_coding No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available