IKMC Project Report - EUCOMM (ID: 117145)

Fkbp11 MGI:1913370 ENSMUSG00000003355
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout-First - Reporter Tagged Insertion'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000003445 protein_coding Residual N-terminal, novel C-terminal product view

Predicted translation: 

MTLSPLLLPLQLLLLLLFSGAVCRAEAGPETESPVRTLQVETLVQPPESCTESAAIGDTLHIHYTGSLVDGRIIDTSLTR
DPLVIELGQKQVIPERSEEQSFLLTWLMESEGTRHLSQPMQWCSTMWS*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000132535 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000132539 PPIase_FKBP_dom (16/93 aa) CDS CDS
ENSMUSE00000132540 PPIase_FKBP_dom (30/93 aa) CDS CDS
ENSMUSE00000132536 PPIase_FKBP_dom (12/93 aa) CDS Deleted
ENSMUSE00000132537 PPIase_FKBP_dom (25/93 aa) CDS CDS
ENSMUSE00000401953 PPIase_FKBP_dom (13/93 aa) CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_H07 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_F12 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_F03 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_E03 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_F05 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_F06 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_B12 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_F02 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_C04 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_D01 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_H10 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_F04 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_C09 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 446430 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00282_Z_3_E10 L1L2_GT1_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
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Intermediate Vectors no data available