IKMC Project Report - EUCOMM (ID: 117314)

0610030E20Rik MGI:1915614 ENSMUSG00000058706
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - No QC Positives

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000077783 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation:

MEAAQDHGPGLCCKPGGRLDMSHGFVHHIRRNQLDRDDYDKKVKQAAKEKARRRHTPAPTRPRKPDLQVYLPRHR

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000459544 UPF0561 (34/125 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000459533 UPF0561 (41/125 aa) CDS CDS + stop codon + UTR
ENSMUSE00000459527 UPF0561 (51/125 aa) CDS Deleted
ENSMUSE00000465273 UPF0561 (1/125 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors

Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 433624 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) HTGR04032_Y_8_C02 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
order 433624 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) HTGR04032_Y_8_B05 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
show/hide more targeting vectors

Intermediate Vectors

Design ID Vector Type Intermediate Vector
433624 Knockout First, Reporter-tagged insertion with conditional potential PCS04032_A_H08