IKMC Project Report - EUCOMM (ID: 117447)
1600016N20Rik MGI:1919250 ENSMUSG00000025500
Mice no data available
Mutagenesis Predictions
This gene has 2 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.
| Ensembl transcript id | Ensembl Biotype | Floxed transcript description | Details | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| ENSMUST00000170841 | protein_coding | Residual N-terminal, residual C-terminal product | view | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Predicted translation: MAPKSCQESEDKQVSPAPAGVQPDSSDLGSPVGTPVDRVAPSYSQSAKLYTSTPMGCSVKQQLAPETLDPRTLRLLWEQR ELEIQALRWAVQNGHNARYSSILQEVAGVPSERLRGMIAPPTRNSKSQDKFLRNQVQKLTLELKAQKEQAQQEKQQLEEK LQQNLWAKQQLEAELQTFQKSCLLQLARSSWVGRVLRSQTGSVEVVTTEVLRDPSDFSESAEIPTSGEGFPLEDVDWNSI AQRYPNLFSNLNFYSDQKQSQPPTSETYTLDSEGATKHTEKPTKILEWSALPLLDLCKSHSPSCSKTVLESYTDLHHPYT RPQLNPFGCCLKIAAVSHREKFIRVINQSQAETIDLGGFVLQQLVRDFPVCMYRFPPGTLLAPQHHITVWGEGTSRTKKQ LPVASGQDPFQFQSSRGCVTVLVNPQGQVLSEHQATPCVTLGSKIFTDNTDWSIDCFPLSESEPDVHPGEQQCRPSSPQK GRAKDAGARRKKPGPGVRQHRHSSTSGLRASRTLHPTETRDILPLLSSRKLLPSGEVLTQQEGVKAETSELLPVIPECPS RLCLGEDSLGRQEYKVQVCRKSVDLSCPMVALSVQNTAESRYGFRFLCYPPITEELCRRL*
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| ENSMUST00000026573 | protein_coding | Residual N-terminal, residual C-terminal product | view | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Predicted translation: MAPKSCQESEDKQVSPAPAGVQPDSSDLGSPVGTPVDRVAPSYSQSAKLYTSTPMGCSVKQQLAPETLDPRTLRLLWEQR ELEIQALRWAVQNGHNARYSSILQEVAGVPSERNSKSQDKFLRNQVQKLTLELKAQKEQAQQEKQQLEEKLQQNLWAKQQ LEAELQTFQKSCLLQLARSSWVGRVLRSQTGSVEVVTTEVLRDPSDFSESAEIPTSGEGFPLEDVDWNSIAQRYPNLFSN LNFYSDQKQSQPPTSETYTLDSEGATKHTEKPTKILEWSALPLLDLCKSHSPSCSKTVLESYTDLHHPYTRPQLNPFGCC LKIAAVSHREKFIRVINQSQAETIDLGGFVLQQLVRDFPVCMYRFPPGTLLAPQHHITVWGEGTSRTKKQLPVASGQDPF QFQSSRGCVTVLVNPQGQVLSEHQATPCVTLGSKIFTDNTDWSIDCFPLSESEPDVHPGEQQCRPSSPQKGRAKDAGARR KKPGPGVRQHRHSSTSGLRASRTLHPTETRDILPLLSSRKLLPSGEVLTQQEGVKAETSELLPVIPECPSRLCLGEDSLG RQEYKVQVCRKSVDLSCPMVALSVQNTAESRYGFRFLCYPPITEELCRRL*
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ES Cell Clones With Knockout First Mutation (Reporter Tagged Insertion With Conditional Potential)
| Genome Browsers: | Ensembl (mouse) - UCSC (mouse) |
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| Tools: | Southern Blot Tool - LRPCR Genotyping Primers |
| ES Cell Clone | Targeting Vector | Allele | Allele Type | Parental ES Cell Line | Genbank File | Mouse | QC Data | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_C06 | ETPG00277_Z_7_G01 | 1600016N20Riktm1a(EUCOMM)Hmgu | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_B05 | ETPG00277_Z_7_G01 | 1600016N20Riktm1a(EUCOMM)Hmgu | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_A04 | ETPG00277_Z_7_G01 | 1600016N20Riktm1a(EUCOMM)Hmgu | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_D04 | ETPG00277_Z_7_G01 | 1600016N20Riktm1a(EUCOMM)Hmgu | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_H04 | ETPG00277_Z_7_G01 | 1600016N20Riktm1a(EUCOMM)Hmgu | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_E06 | ETPG00277_Z_7_G01 | 1600016N20Riktm1a(EUCOMM)Hmgu | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_G06 | ETPG00277_Z_7_G01 | 1600016N20Riktm1a(EUCOMM)Hmgu | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_F05 | ETPG00277_Z_7_G01 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_D06 | ETPG00277_Z_7_G01 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_A05 | ETPG00277_Z_7_G01 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_A06 | ETPG00277_Z_7_G01 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_F06 | ETPG00277_Z_7_G01 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | HEPD0850_4_C05 | ETPG00277_Z_7_G01 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8.N4 | view | no | view ( about ) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Targeting Vectors
| Genome Browsers: | Ensembl (mouse) - UCSC (mouse) |
|---|---|
| Tools: | LRPCR Genotyping Primers |
| Design ID | Vector Type | Targeting Vector | Cassette | Backbone | Genbank File | |
|---|---|---|---|---|---|---|
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_G01 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_X_6_F03 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_B10 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_A02 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_A12 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_H10 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_A10 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_A09 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_C10 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_F02 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_A07 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_X_6_E10 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_X_6_G01 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_E05 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_X_6_E06 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_C02 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_E03 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_E01 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_B06 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_G08 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_E11 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_H09 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_B05 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |
| order | 428772 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | ETPG00277_Z_7_G10 | Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo | L3L4_pD223_DTA_T_spec | view |