IKMC Project Report - EUCOMM (ID: 117731)

Ffar4 MGI:2147577 ENSMUSG00000054200
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - Targeting Confirmed

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout-First - Reporter Tagged Insertion'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000067098 protein_coding Residual N-terminal, residual C-terminal product view

Predicted translation: 

MSPECAQTTGPGPSHTLDQVNRTHFPFFSDVKGDHRLVLSVVEEIPICTLDWPNRIGEISWDVFFVTLNFLVPGLVIVIS
YSKILQITKASRKRLTLSLAYSESHQIRVSQQDYRLFRTLFLLMVSFFIMWSPIIITILLILIQNFRQDLVIWPSLFFWV
VAFTFANSALNPILYNMSLFRNEWRKIFCCFFFPEKGAIFTDTSVRRNDLSVISS*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000452038 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000452038 7TM_GPCR_Rhodpsn (132/265 aa) CDS Deleted
ENSMUSE00000454623 7TM_GPCR_Rhodpsn (43/265 aa) CDS CDS
ENSMUSE00000452036 7TM_GPCR_Rhodpsn (90/265 aa) CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones Without Conditional Potential

Note: Mutations of type "Without Conditional Potential" are correctly targeted clones that have lost the 3' LoxP site. These mutations cannot be converted into conditional alleles.

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool - LRPCR Genotyping Primers
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order HEPD0850_2_H01 ETPG00277_Y_1_D09 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) JM8.N4 view no view    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order HEPD0850_2_B01 ETPG00277_Y_1_D09 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) JM8.N4 view no view    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
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Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_Y_1_D09 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_A08 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_C04 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_F07 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_E05 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_F11 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_C02 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_A03 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_G05 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_C04 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_G09 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_G10 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_A07 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_Y_4_C08 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_F09 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_F01 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_G08 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_F05 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_H05 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_E12 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_D08 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_B05 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_E06 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_E07 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_E04 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_C05 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_E01 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_E10 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_1_D04 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_D04 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_Y_1_E04 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_H08 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_G10 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_F12 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_C05 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_G03 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 435270 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) ETPG00277_X_2_E03 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
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Intermediate Vectors no data available