IKMC Project Report - EUCOMM (ID: 117760)

Tmem259 MGI:2177957 ENSMUSG00000013858 OTTMUSG00000019134
Pre-pipeline Designs Vectors ES Cells Mice
Design Completed

Mice no data available

Mutagenesis Predictions

This gene has 5 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000052885 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation:

MSEHAAAPGPGPNGGGGGGAAPVRGPRGPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLF
VLAYIHIVFSRSPINCLEHVRDRWPREGVLRVEVRHNSSRAPVILQFCDGGLGGLELEPGGLELEEEELTVEMFTNSSIK
FELDIEPKVFKPQSGADALNDSQDFPFPETPAKVWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGD
RFSRLLLDEFLGYDDILMSSVKGLAENEENK

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000371876 Membralin (41/361 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000369486 Membralin (86/361 aa) CDS CDS
ENSMUSE00000100943 Membralin (34/361 aa) CDS CDS
ENSMUSE00000100936 Membralin (38/361 aa) CDS CDS
ENSMUSE00000100939 Membralin (42/361 aa) CDS CDS + stop codon + UTR
ENSMUSE00001033090 Membralin (34/361 aa) CDS Deleted
ENSMUSE00001071549 Membralin (20/361 aa) CDS Deleted
ENSMUSE00001005707 Membralin (29/361 aa) CDS Deleted
ENSMUSE00000100940 Membralin (42/361 aa) CDS Deleted
ENSMUSE00001042786 CDS Deleted
ENSMUSE00000786777 CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000124536 protein_coding No analysis available: incomplete transcript
ENSMUST00000132080 retained_intron No analysis available: incomplete transcript
ENSMUST00000126383 retained_intron No analysis available: incomplete transcript
ENSMUST00000124889 retained_intron No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available