IKMC Project Report - EUCOMM (ID: 118225)

Xdh MGI:98973 ENSMUSG00000024066
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - No QC Positives

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000024866 protein_coding No protein product (NMD) view

Predicted translation:

MTRTTVDELVFFVNGKKWGCAGPSLAVEKVAVGHAP*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000230729 2Fe-2S_ferredoxin-type (5/69 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000137922 2Fe-2S_ferredoxin-type (20/69 aa) CDS Deleted
ENSMUSE00000137888 2Fe-2S_ferredoxin-type (33/69 aa) CDS CDS + stop codon + UTR
ENSMUSE00000137873 2Fe-2S-bd (14/73 aa) | 2Fe-2S_ferredoxin-type (13/69 aa) CDS
ENSMUSE00000137846 2Fe-2S-bd (43/73 aa) CDS
ENSMUSE00000137886 2Fe-2S-bd (17/73 aa) CDS
ENSMUSE00000137855 CDS
ENSMUSE00000137912 CDS
ENSMUSE00000137856 Mopterin_DH_FAD-bd (32/179 aa) CDS
ENSMUSE00000137851 Mopterin_DH_FAD-bd (32/179 aa) CDS
ENSMUSE00000137911 Mopterin_DH_FAD-bd (51/179 aa) CDS
ENSMUSE00000137872 Mopterin_DH_FAD-bd (32/179 aa) CDS
ENSMUSE00000137887 Mopterin_DH_FAD-bd (35/179 aa) CDS
ENSMUSE00000137875 CO_DH_flav_C (57/105 aa) CDS
ENSMUSE00000230452 CO_DH_flav_C (49/105 aa) CDS
ENSMUSE00000230431 CDS
ENSMUSE00000137896 Ald_Oxase/Xan_DH_a/b (31/107 aa) CDS
ENSMUSE00000230377 Ald_Oxase/Xan_DH_a/b (42/107 aa) CDS
ENSMUSE00000230347 Ald_Oxase/Xan_DH_a/b (35/107 aa) CDS
ENSMUSE00000230325 AldOxase/xan_DH_Mopterin-bd (31/536 aa) CDS
ENSMUSE00000230305 AldOxase/xan_DH_Mopterin-bd (42/536 aa) CDS
ENSMUSE00000230286 AldOxase/xan_DH_Mopterin-bd (45/536 aa) CDS
ENSMUSE00000230268 AldOxase/xan_DH_Mopterin-bd (30/536 aa) CDS
ENSMUSE00000230247 AldOxase/xan_DH_Mopterin-bd (29/536 aa) CDS
ENSMUSE00000137910 AldOxase/xan_DH_Mopterin-bd (64/536 aa) CDS
ENSMUSE00000137871 AldOxase/xan_DH_Mopterin-bd (49/536 aa) CDS
ENSMUSE00000137848 AldOxase/xan_DH_Mopterin-bd (28/536 aa) CDS
ENSMUSE00000137882 AldOxase/xan_DH_Mopterin-bd (32/536 aa) CDS
ENSMUSE00000137917 AldOxase/xan_DH_Mopterin-bd (43/536 aa) CDS
ENSMUSE00000137903 AldOxase/xan_DH_Mopterin-bd (25/536 aa) CDS
ENSMUSE00000137898 AldOxase/xan_DH_Mopterin-bd (18/536 aa) CDS
ENSMUSE00000137890 AldOxase/xan_DH_Mopterin-bd (39/536 aa) CDS
ENSMUSE00000137899 AldOxase/xan_DH_Mopterin-bd (22/536 aa) CDS
ENSMUSE00000137897 AldOxase/xan_DH_Mopterin-bd (43/536 aa) CDS
ENSMUSE00000137880 CDS
ENSMUSE00000341621 CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors

Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 428624 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00276_Y_1_H06 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
order 428624 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00276_Y_H06 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
order 428624 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00276_Z_1_B02 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
order 428624 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00276_Z_1_H06 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
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Intermediate Vectors no data available