IKMC Project Report - KOMP-CSD (ID: 118285)

Tgm4 MGI:3027002 ENSMUSG00000025787 OTTMUSG00000023752
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 4 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000026893 protein_coding No protein product (NMD) view

Predicted translation:

MDSRNVLIIYAVNVERKLNAAAHHTSEYQTKKLVLRRGQIFTLKVILNRPLQPQDELKVTFTSGYSECHQCC*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00001006593 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001048603 Transglutaminase_N (56/110 aa) CDS CDS
ENSMUSE00001003731 Transglutaminase_N (33/110 aa) CDS Deleted
ENSMUSE00000976055 Transglutaminase_N (22/110 aa) CDS CDS + stop codon + UTR
ENSMUSE00000995208 CDS
ENSMUSE00000254000 CDS
ENSMUSE00000153192 Transglutaminase-like (15/88 aa) CDS
ENSMUSE00001090627 Transglutaminase-like (47/88 aa) CDS
ENSMUSE00000153200 Transglutaminase-like (28/88 aa) CDS
ENSMUSE00000361104 CDS
ENSMUSE00000153195 CDS
ENSMUSE00000153199 Transglutaminase_C (4/96 aa) CDS
ENSMUSE00000153197 Transglutaminase_C (46/96 aa) CDS
ENSMUSE00000343342 Transglutaminase_C (47/96 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000128572 retained_intron No analysis available: incomplete transcript
ENSMUST00000143364 processed_transcript Target region does not overlap transcript
ENSMUST00000171897 processed_transcript No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available