IKMC Project Report - (ID: 118320)

Pre-pipeline Designs Vectors ES Cells Mice

Mice no data available

Mutagenesis Predictions

This gene has 5 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Cre Knock In'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000021273 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation:

MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDE

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000831110 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000498763 Synaptobrevin (13/87 aa) CDS CDS
ENSMUSE00001074748 Synaptobrevin (53/87 aa) CDS Deleted
ENSMUSE00000398924 Synaptobrevin (18/87 aa) CDS Deleted
ENSMUSE00000360976 Synaptobrevin (4/87 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000117780 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation:

MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDE

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000714048 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000498763 Synaptobrevin (13/84 aa) CDS CDS
ENSMUSE00001074748 Synaptobrevin (53/84 aa) CDS Deleted
ENSMUSE00000712032 Synaptobrevin (18/84 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000149559 processed_transcript No analysis available: incomplete transcript
ENSMUST00000149780 retained_intron No analysis available: incomplete transcript
ENSMUST00000146847 retained_intron No analysis available: incomplete transcript
show/hide more transcripts

ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
currently unavailable 46246 Cre Knock In (Promotorless Cassette) ETPG0003_Z_2_H09 pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro L3L4_pD223_DTA_T_spec view
currently unavailable 46246 Cre Knock In (Promotorless Cassette) ETPG0003_Z_2_B09 pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro L3L4_pD223_DTA_T_spec view
currently unavailable 46246 Cre Knock In (Promotorless Cassette) ETPG0003_Z_2_D05 pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro L3L4_pD223_DTA_T_spec view
show/hide more targeting vectors

Intermediate Vectors

Design ID Vector Type Intermediate Vector
46246 Cre Knock In PCS00060_A_E09