IKMC Project Report - KOMP-CSD (ID: 118386)

Hoxa3 MGI:96175 ENSMUSG00000079560 OTTMUSG00000018880
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 4 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000114434 protein_coding No protein product
ENSMUST00000134831 protein_coding No analysis available: incomplete transcript
ENSMUST00000142764 retained_intron Target region does not overlap transcript
ENSMUST00000148446 processed_transcript Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 87866 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MOHFA0006_A_6_A03 L1L2_gt0_Del_LacZ R3R4_pBR_amp view
order 87866 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MOHFA0006_A_4_A03 L1L2_gt0_Del_LacZ R3R4_pBR_amp view
order 87866 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MOHFA0006_A_5_A04 L1L2_gt0_Del_LacZ R3R4_pBR_amp view
order 87866 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MOHFA0006_A_5_A03 L1L2_gt0_Del_LacZ R3R4_pBR_amp view
order 87866 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MOHFA0006_A_6_A04 L1L2_gt0_Del_LacZ R3R4_pBR_amp view
order 87866 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MOHFA0006_A_8_A04 L1L2_gt0_Del_LacZ R3R4_pBR_amp view
order 87866 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MOHFA0006_A_3_A03 L1L2_gt0_Del_LacZ R3R4_pBR_amp view
order 87866 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MOHFA0006_A_8_A03 L1L2_gt0_Del_LacZ R3R4_pBR_amp view
order 87866 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MOHFA0006_A_2_A03 L1L2_gt0_Del_LacZ R3R4_pBR_amp view
order 87866 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MOHFA0006_A_1_A03 L1L2_gt0_Del_LacZ R3R4_pBR_amp view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
87866 Knockout First, Reporter-tagged insertion with conditional potential MOHPC10006_A_A03
87866 Knockout First, Reporter-tagged insertion with conditional potential MOHPC10006_A_A04