IKMC Project Report - EUCOMM (ID: 120479)

Katnbl1 MGI:1919675 ENSMUSG00000027132 OTTMUSG00000015139
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000028552 protein_coding No protein product (NMD) view

Predicted translation:

MAFDTHHVKKRNFSNSIDLPRKRISNFTSKNMKEVKRSPKQLAAYISRFLRTMRQWPRFYSAGI*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000788585 UTR UTR
ENSMUSE00001062486 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001041083 CDS CDS
ENSMUSE00000166387 CDS Deleted
ENSMUSE00000166388 CDS CDS + stop codon + UTR
ENSMUSE00000166383 CDS
ENSMUSE00000166385 CDS
ENSMUSE00000312737 CDS
ENSMUSE00000312730 CDS
ENSMUSE00000369808 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000151257 retained_intron No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors

Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 378518 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) HTGR04029_Z_3_D04 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view

Intermediate Vectors

Design ID Vector Type Intermediate Vector
378518 Knockout First, Reporter-tagged insertion with conditional potential PCS04029_A_C09