IKMC Project Report - EUCOMM (ID: 120595)

Opn3 MGI:1338022 ENSMUSG00000026525 OTTMUSG00000021478
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - Electroporation Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000027809 protein_coding No protein product (NMD) view

Predicted translation:

MYSGNRSGDQGYWEDGAGAEGAAPAGTRSPAPLFSPTAYERLALLLGCLALLGVGGNLLVLLLYSKFPRLRTPTHLFLVN
LSLGDLLVSLFGVTFTFASCLRNGWVWDAVGCAWDGFSGSLFASLC*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000398686 7TM_GPCR_Rhodpsn (67/252 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000323080 7TM_GPCR_Rhodpsn (107/252 aa) CDS Deleted
ENSMUSE00000160211 7TM_GPCR_Rhodpsn (79/252 aa) CDS CDS + stop codon + UTR
ENSMUSE00000400299 CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available