IKMC Project Report - EUCOMM (ID: 24006)

Depdc7 MGI:2139258 ENSMUSG00000027173 OTTMUSG00000014963
Pre-pipeline Designs Vectors ES Cells Mice
Vector Unsuccessful - Project Terminated

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000028595 protein_coding No protein product (NMD) view

Predicted translation:

MATVREKAAALNLSALHSPAQRPPGTQMHRLCHLISSRTVWRTCGRT*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000166543 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000166547 DEP_dom (85/85 aa) CDS Deleted
ENSMUSE00000166542 CDS CDS + stop codon + UTR
ENSMUSE00000166549 CDS
ENSMUSE00000166545 CDS
ENSMUSE00000166548 CDS
ENSMUSE00000166544 CDS
ENSMUSE00000166546 CDS
ENSMUSE00000406652 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000144133 retained_intron Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available