IKMC Project Report - EUCOMM (ID: 24410)

Tbx21 MGI:1888984 ENSMUSG00000001444 OTTMUSG00000002233
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000001484 protein_coding No protein product (NMD) view

Predicted translation:

MGIVEPGCGDMLTGTEPMPSDEGRGPGADQQHRFFYPEPGAQDPTDRRAGSSLGTPYSGGALVPAAPGRFLGSFAYPPRA
QVAGFPGPGEFFPPPAGAEGYPPVDGYPAPDPRAGLYPGPREDYALPAGLEVSGKLRVALSNHLLWSKFNQHQTEMIITK
QGR*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000390115 TF_T-box (25/189 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000287808 TF_T-box (53/189 aa) CDS Deleted
ENSMUSE00000287801 TF_T-box (41/189 aa) CDS Deleted
ENSMUSE00000287790 TF_T-box (53/189 aa) CDS CDS + stop codon + UTR
ENSMUSE00000134561 TF_T-box (19/189 aa) CDS
ENSMUSE00000333991 CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available