IKMC Project Report - KOMP-CSD (ID: 24901)

Itih5 MGI:1925751 ENSMUSG00000025780 OTTMUSG00000011192
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - Electroporation Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000026886 protein_coding No protein product (NMD) view

Predicted translation:

MLLLLGLCLGLPLFSESQEEARSWDDTSEQVVLRVPRQLRLLQRLKTKPLMAEFSVKSTIISRYAFTTVSCRMLNRASED
QEAEFQMQIPESAFITNFTMLIGDSVYRGEITQKDKKSSESVKDKRNRTSDDNE*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000341745 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000153146 CDS CDS
ENSMUSE00000153137 VIT (52/114 aa) CDS CDS
ENSMUSE00000153143 VIT (35/114 aa) CDS CDS
ENSMUSE00000153147 VIT (29/114 aa) CDS Deleted
ENSMUSE00000153131 CDS CDS + stop codon + UTR
ENSMUSE00000153129 VWF_A (18/165 aa) CDS
ENSMUSE00000153148 VWF_A (57/165 aa) CDS
ENSMUSE00000153142 VWF_A (91/165 aa) CDS
ENSMUSE00000153140 CDS
ENSMUSE00000153130 CDS
ENSMUSE00000153132 ITI_HC_C (2/195 aa) CDS
ENSMUSE00000153127 ITI_HC_C (127/195 aa) CDS
ENSMUSE00000343752 ITI_HC_C (68/195 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available