IKMC Project Report - EUCOMM (ID: 25490)

Trafd1 MGI:1923551 ENSMUSG00000042726 OTTMUSG00000026315
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 8 wild type transcripts, of which 5 are protein-coding. Following removal of the floxed region, 5 transcripts are predicted to produce a truncated protein product of which 2 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000042312 protein_coding No protein product (NMD) view

Predicted translation:

MAEFRDDQASRLCDNCKKEIPVFNFTIHEIHCQRNIGVCPVCKEPFPKSDMDIHMAAEHCQVTCKCNKKLEKRQLKQHAL
KNKKGRKGTEAGSPQRTALRITHTWTSCWP*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000733778 UTR UTR
ENSMUSE00000407356 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000358935 CDS CDS
ENSMUSE00000399746 CDS CDS
ENSMUSE00000361672 CDS Deleted
ENSMUSE00001006072 CDS CDS + stop codon + UTR
ENSMUSE00000405266 CDS
ENSMUSE00000283590 CDS
ENSMUSE00000283585 CDS
ENSMUSE00000353669 CDS
ENSMUSE00000997866 CDS
ENSMUSE00001059184 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000120784 protein_coding No protein product (NMD) view

Predicted translation:

MAEFRDDQASRLCDNCKKEIPVFNFTIHEIHCQRNIGVCPVCKEPFPKSDMDIHMAAEHCQVTCKCNKKLEKRQLKQHAL
KNKKGRKGTEAGSPQRTALRITHTWTSCWP*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000716503 UTR UTR
ENSMUSE00000407356 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000358935 CDS CDS
ENSMUSE00000399746 CDS CDS
ENSMUSE00000361672 CDS Deleted
ENSMUSE00001006072 CDS CDS + stop codon + UTR
ENSMUSE00000405266 CDS
ENSMUSE00000283590 CDS
ENSMUSE00000283585 CDS
ENSMUSE00000712363 CDS
ENSMUSE00000997866 CDS
ENSMUSE00000709550 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000152265 protein_coding No analysis available: incomplete transcript
ENSMUST00000155379 protein_coding No analysis available: incomplete transcript
ENSMUST00000146185 protein_coding Target region does not overlap transcript
ENSMUST00000156158 retained_intron No analysis available: incomplete transcript
ENSMUST00000133138 retained_intron Target region does not overlap transcript
ENSMUST00000126550 processed_transcript Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available