IKMC Project Report - KOMP-CSD (ID: 27395)

Ints2 MGI:1917672 ENSMUSG00000018068 OTTMUSG00000001206
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 11 wild type transcripts, of which 5 are protein-coding. Following removal of the floxed region, 5 transcripts are predicted to produce a truncated protein product of which 2 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000018212 protein_coding No protein product (NMD) view

Predicted translation:

MTPEGTGLQFVSPFAFEAMQKVDVVRLASLSDPELRLLLPCLVRMALCAPADQSQSWAQDKKLILRLLSGVEAVNSIVAL
LSVDFHALEQDASKEQQLRHKLGGGSGESILVSQLQHGLTLEFEHSDSPRRLRLVLSELLAIMNKSSLPCFL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000415440 UTR UTR
ENSMUSE00001020178 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001053947 CDS CDS
ENSMUSE00000996003 CDS Deleted
ENSMUSE00001082463 CDS CDS + stop codon + UTR
ENSMUSE00001061427 CDS
ENSMUSE00000988569 CDS
ENSMUSE00000362322 CDS
ENSMUSE00000460100 CDS
ENSMUSE00000969720 CDS
ENSMUSE00000977288 CDS
ENSMUSE00000991376 CDS
ENSMUSE00001024986 CDS
ENSMUSE00000460062 CDS
ENSMUSE00000460057 CDS
ENSMUSE00000979759 CDS
ENSMUSE00001092513 CDS
ENSMUSE00000966069 CDS
ENSMUSE00000987649 CDS
ENSMUSE00000333600 CDS
ENSMUSE00000105622 CDS
ENSMUSE00000105625 CDS
ENSMUSE00000105621 CDS
ENSMUSE00000105623 CDS
ENSMUSE00000271659 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000108039 protein_coding No protein product (NMD) view

Predicted translation:

MTPEGTGLQFVSPFAFEAMQKVDVVRLASLSDPELRLLLPCLVRMALCAPADQSQSWAQDKKLILRLLSGVEAVNSIVAL
LSVDFHALEQDASKEQQLRHKLGGGSGESILVSQLQHGLTLEFEHSDSPRRLRLVLSELLAIMNKSSLPCFL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000675313 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001053947 CDS CDS
ENSMUSE00000996003 CDS Deleted
ENSMUSE00001082463 CDS CDS + stop codon + UTR
ENSMUSE00001061427 CDS
ENSMUSE00000988569 CDS
ENSMUSE00000362322 CDS
ENSMUSE00000460100 CDS
ENSMUSE00000969720 CDS
ENSMUSE00000977288 CDS
ENSMUSE00000991376 CDS
ENSMUSE00001024986 CDS
ENSMUSE00000460062 CDS
ENSMUSE00000460057 CDS
ENSMUSE00000979759 CDS
ENSMUSE00001092513 CDS
ENSMUSE00000966069 CDS
ENSMUSE00000987649 CDS
ENSMUSE00000333600 CDS
ENSMUSE00000105622 CDS
ENSMUSE00000105625 CDS
ENSMUSE00000105621 CDS
ENSMUSE00000105623 CDS
ENSMUSE00000675312 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000139285 protein_coding No analysis available: incomplete transcript
ENSMUST00000132024 protein_coding Target region does not overlap transcript
ENSMUST00000136469 protein_coding Target region does not overlap transcript
ENSMUST00000134828 nonsense_mediated_decay Target region does not overlap transcript
ENSMUST00000134883 retained_intron No analysis available: incomplete transcript
ENSMUST00000127745 retained_intron Target region does not overlap transcript
ENSMUST00000146421 retained_intron Target region does not overlap transcript
ENSMUST00000141756 processed_transcript Target region does not overlap transcript
ENSMUST00000130614 retained_intron Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available