IKMC Project Report - EUCOMM (ID: 27680)

Wipi1 MGI:1261864 ENSMUSG00000041895 OTTMUSG00000003347
Pre-pipeline Designs Vectors ES Cells Mice
Vector Unsuccessful - Project Terminated

Mice no data available

Mutagenesis Predictions

This gene has 4 wild type transcripts, of which 3 are protein-coding. Following removal of the floxed region, 3 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000103060 protein_coding Residual C-terminal product view

Predicted translation: 

MPLRAEWRRRSAASLSTKTAHEIPDVYIVERLFSSSLVVVVSHTKPRQMNVYHFKKGTEICNYSYSSNILSIRLNRQRLL
VCLEESIYIHNIKDMKLLKTVLDIPSNPTGLCALSINHSNSYLAYPGSQSTGEIVLYDGNSLKTVCTIAAHEGTLAAITF
NSSGSKLASASEKGTVIRVFSVPEGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFKMEHLTDSRPEEPST
WSGYMGKMFMAATNYLPAQVSDMMNQDRAFATGRLNFSGQKNICTLSTIQKLPRLLVASSDGHLYIYNLDPQDGGECVLI
KTHSLLSSGTTEENKENDLRPSLPPSYAATVARPSTSAASTVPGYSEDGGALRGEVIPEHEFATGPVCLDDENEFPPIIL
CRGSQKGKTKQS*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000661146 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000233267 CDS Deleted
ENSMUSE00000233262 CDS CDS
ENSMUSE00000233258 CDS CDS
ENSMUSE00000233251 CDS CDS
ENSMUSE00000233245 WD40_repeat (28/32 aa) CDS CDS
ENSMUSE00000233238 WD40_repeat (4/32 aa) CDS CDS
ENSMUSE00000661145 CDS CDS
ENSMUSE00000661144 CDS CDS
ENSMUSE00000233214 CDS CDS
ENSMUSE00000233208 CDS CDS
ENSMUSE00000233198 CDS CDS
ENSMUSE00000661143 CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000047186 protein_coding Residual C-terminal product view

Predicted translation: 

MPLRAEWRRRSAASLSTKTAHEIPDVYIVERLFSSSLVVVVSHTKPRQMNVYHFKKGTEICNYSYSSNILSIRLNRQRLL
VCLEESIYIHNIKDMKLLKTVLDIPSNPTGLCALSINHSNSYLAYPGSQSTGEIVLYDGNSLKTVCTIAAHEGTLAAITF
NSSGSKLASASEKGTVIRVFSVPEGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFKMEHLTDSRPEEPST
WSGYMGKMFMAATNYLPAQVSDMMNQDRAFATGRLNFSGQKNICTLSTIQKLPRLLVASSDGHLYIYNLDPQDGGECVLI
KTHSLLSSGTTEENKENDLRPSLPPSYAATVARPSTSAASTVPGYSEDGGALRGEVIPEHEFATGPVCLDDENEFPPVSI
RNP*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000661146 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000233267 CDS Deleted
ENSMUSE00000233262 CDS CDS
ENSMUSE00000233258 CDS CDS
ENSMUSE00000233251 CDS CDS
ENSMUSE00000233245 WD40_repeat (28/32 aa) CDS CDS
ENSMUSE00000233238 WD40_repeat (4/32 aa) CDS CDS
ENSMUSE00000661145 CDS CDS
ENSMUSE00000661144 CDS CDS
ENSMUSE00000233214 CDS CDS
ENSMUSE00000233208 CDS CDS
ENSMUSE00000400352 CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000106689 protein_coding Residual C-terminal product view

Predicted translation: 

MPLRAEWRRRSAASLSTKTAHEIPDVYIVERLFSSSLVVVVSHTKPRQMNVYHFKKGTEICNYSYSSNILSIRLNRQRLL
VCLEESIYIHNIKDMKLLKTVLDIPSNPTGLCALSINHSNSYLAYPGSQSTGEIVLYDGNSLKTVCTIAAHEGTLAAITF
NSSGSKLASASEKGTVIRVFSVPEGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFKMEHLTDRQVRGQAE
FLPDGRSSPRLFCVRSLELHF*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000661146 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000233267 CDS Deleted
ENSMUSE00000233262 CDS CDS
ENSMUSE00000233258 CDS CDS
ENSMUSE00000233251 CDS CDS
ENSMUSE00000233245 WD40_repeat (28/32 aa) CDS CDS
ENSMUSE00000233238 WD40_repeat (4/32 aa) CDS CDS
ENSMUSE00000233228 CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000153738 processed_transcript Target region does not overlap transcript
show/hide more transcripts

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available