IKMC Project Report - EUCOMM (ID: 29007)

Dhcr24 MGI:1922004 ENSMUSG00000034926 OTTMUSG00000008196
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000047973 protein_coding No protein product (NMD) view

Predicted translation:

MEPAVSLAVCALLFLLWVRVKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRLHEQRVRDIQKQVRE
WKEQGSKTFMCTGRPGWLTVSLRVGKYKKTHKNIMINLMDILEVDTKKQGA*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000366461 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000471216 Oxid_FAD_bind_N (17/92 aa) CDS CDS
ENSMUSE00000274899 Oxid_FAD_bind_N (36/92 aa) CDS Deleted
ENSMUSE00000274893 Oxid_FAD_bind_N (40/92 aa) CDS CDS + stop codon + UTR
ENSMUSE00000274886 CDS
ENSMUSE00000150610 CDS
ENSMUSE00000150608 CDS
ENSMUSE00000274873 CDS
ENSMUSE00000508157 CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available