IKMC Project Report - KOMP-CSD (ID: 29603)

I0C0044D17Rik MGI:3588230 ENSMUSG00000073794 OTTMUSG00000025486
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000097964 protein_coding No protein product
ENSMUST00000168045 protein_coding Design floxes first exon in transcript ENSMUST00000168045
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ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 221071 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) HTGRS6003_A_F12 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 221071 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) HTGR06003_A_1_F12 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 221071 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) HTGR06003_A_1_G12 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
221071 Knockout First, Reporter-tagged insertion with conditional potential PCS6003_A_F12