IKMC Project Report - EUCOMM (ID: 29826)

Col6a1 MGI:88459 ENSMUSG00000001119 OTTMUSG00000020489
Pre-pipeline Designs Vectors ES Cells Mice
Vector Construction in Progress

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000001147 protein_coding No protein product (NMD) view

Predicted translation:

MRLAHALLPLLLQACWVATQDIQGSKAIAFQDCPVDLFFVLDTSESVALRLKPYGALVDKVKSFTKRFIDNLRDRYYRCD
RNLVWNAGALHYSDEVEIIRGLTRMPSGRDELKASVDAVKYFGKGTYTDCAIKKGLEELLIGGSEESQVFRERREKLETL
DDLGILDQSGTRV*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000838593 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001052521 VWF_A (39/176 aa) CDS CDS
ENSMUSE00001027526 VWF_A (68/176 aa) CDS CDS
ENSMUSE00000978270 VWF_A (54/176 aa) CDS Deleted
ENSMUSE00001088163 VWF_A (17/176 aa) CDS Deleted
ENSMUSE00001090588 CDS Deleted
ENSMUSE00000993350 CDS Deleted
ENSMUSE00001017528 Collagen (11/56 aa) CDS Deleted
ENSMUSE00001036728 Collagen (18/56 aa) CDS CDS
ENSMUSE00001062747 Collagen (15/56 aa) CDS CDS + stop codon + UTR
ENSMUSE00001085692 Collagen (9/56 aa) | Collagen (5/56 aa) CDS
ENSMUSE00001028446 Collagen (3/56 aa) | Collagen (9/56 aa) CDS
ENSMUSE00001002550 Collagen (15/56 aa) CDS
ENSMUSE00000999766 Collagen (18/56 aa) CDS
ENSMUSE00001090656 Collagen (9/56 aa) | Collagen (8/57 aa) CDS
ENSMUSE00000986248 Collagen (21/57 aa) CDS
ENSMUSE00001031511 Collagen (18/57 aa) CDS
ENSMUSE00001021477 Collagen (10/57 aa) CDS
ENSMUSE00001067311 CDS
ENSMUSE00000974659 Collagen (8/59 aa) CDS
ENSMUSE00001076543 Collagen (21/59 aa) CDS
ENSMUSE00000966396 Collagen (21/59 aa) | Collagen (8/58 aa) CDS
ENSMUSE00001006563 Collagen (9/59 aa) | Collagen (17/58 aa) CDS
ENSMUSE00000989557 Collagen (12/58 aa) CDS
ENSMUSE00001037194 Collagen (21/58 aa) CDS
ENSMUSE00000983098 CDS
ENSMUSE00001051929 CDS
ENSMUSE00001018073 CDS
ENSMUSE00000998732 CDS
ENSMUSE00000382509 VWF_A (37/162 aa) CDS
ENSMUSE00000102386 VWF_A (37/162 aa) CDS
ENSMUSE00000102404 VWF_A (62/162 aa) CDS
ENSMUSE00000102418 VWF_A (27/162 aa) CDS
ENSMUSE00000102430 CDS
ENSMUSE00000329242 VWF_A (168/168 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000137599 retained_intron Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available