IKMC Project Report - EUCOMM (ID: 30730)

Car4 MGI:1096574 ENSMUSG00000000805 OTTMUSG00000001034
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 6 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000103194 protein_coding Residual N-terminal, novel C-terminal product view

Predicted translation:

MQLLLALLALAYVAPSTEDSGWCYEIQTKDPRSSCLVPGILKKSVL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000105763 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001049199 Carbonic_anhydrase_a (15/256 aa) CDS CDS
ENSMUSE00000970208 Carbonic_anhydrase_a (53/256 aa) CDS Deleted
ENSMUSE00001027092 Carbonic_anhydrase_a (49/256 aa) CDS Deleted
ENSMUSE00001039705 Carbonic_anhydrase_a (27/256 aa) CDS Deleted
ENSMUSE00000105764 Carbonic_anhydrase_a (23/256 aa) CDS Deleted
ENSMUSE00000105772 Carbonic_anhydrase_a (55/256 aa) CDS Deleted
ENSMUSE00000661552 Carbonic_anhydrase_a (37/256 aa) CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000108076 protein_coding No analysis available: incomplete transcript
ENSMUST00000150596 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000127827 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000138331 processed_transcript Target region does not overlap transcript
ENSMUST00000139416 retained_intron Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available