IKMC Project Report - EUCOMM (ID: 30855)

Eif2s3y MGI:1349430 ENSMUSG00000069049 OTTMUSG00000021559
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 7 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000091197 protein_coding No protein product (NMD) view

Predicted translation:

MAGGEAGVTLGQPHLSRQDLATLDVTKLTPLSREIISRQATINIGTIGHVAHGKSTVVKAISGVHTVRFKNELERNITIK
LGYANAKIYKLDDSSCPRPECYRSCGSSTPDEFPSDIPGTKGNFRLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIA
GNESCPQPQTSEHLAAIEIMKLKHILILQNKIDLVKESQAKEQYEQILAFVQGGTRDRSETWYCF*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000773651 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000975241 ProtSyn_GTP-bd (5/205 aa) CDS CDS
ENSMUSE00001020574 ProtSyn_GTP-bd (43/205 aa) CDS CDS
ENSMUSE00001046154 ProtSyn_GTP-bd (41/205 aa) CDS CDS
ENSMUSE00001011787 ProtSyn_GTP-bd (33/205 aa) CDS CDS
ENSMUSE00000980073 ProtSyn_GTP-bd (54/205 aa) CDS CDS
ENSMUSE00000568614 ProtSyn_GTP-bd (33/205 aa) CDS Deleted
ENSMUSE00000568611 Transl_elong_EFTu/EF1A_2 (13/84 aa) CDS Deleted
ENSMUSE00000568610 Transl_elong_EFTu/EF1A_2 (49/84 aa) CDS CDS + stop codon + UTR
ENSMUSE00001057704 TIF2_gsu_C (26/92 aa) | Transl_elong_EFTu/EF1A_2 (23/84 aa) CDS
ENSMUSE00001056881 TIF2_gsu_C (58/92 aa) CDS
ENSMUSE00000789510 TIF2_gsu_C (9/92 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000148961 retained_intron No analysis available: incomplete transcript
ENSMUST00000137006 retained_intron Target region does not overlap transcript
ENSMUST00000144042 retained_intron No analysis available: incomplete transcript
ENSMUST00000134820 retained_intron Target region does not overlap transcript
ENSMUST00000139083 processed_transcript No analysis available: incomplete transcript
ENSMUST00000154556 retained_intron Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available