IKMC Project Report - EUCOMM (ID: 31670)

Rab12 MGI:894284 ENSMUSG00000023460 OTTMUSG00000021023
Pre-pipeline Designs Vectors ES Cells Mice
Vector Unsuccessful - Project Terminated

Mice no data available

Mutagenesis Predictions

This gene has 5 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 2 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000070538 protein_coding No protein product (NMD) view

Predicted translation:

MLLPLLRRSCFPSSTPGGGGGGGSAERVEGARGPGARGRRPEREPYACMDPSAALHRRPAGGSLGAVSPALSGGQARRRK
QPPRPADFKLQVIIIGSRGVGKTSLMERFTDDTFCEACKSTVGTQQGRSDLTALPQPITEVPRGSYSYMTSPRRRRSMTC
QSG*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000823895 Small_GTPase (31/161 aa) | MIRO-like (31/114 aa) | Small_GTPase_ARF/SAR (34/160 aa) | ProtSyn_GTP-bd (30/158 aa) | Gtr1_RagA (31/137 aa) | SRP_receptor_beta_su (32/138 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001066698 Small_GTPase (21/161 aa) | MIRO-like (21/114 aa) | Small_GTPase_ARF/SAR (21/160 aa) | ProtSyn_GTP-bd (21/158 aa) | Gtr1_RagA (21/137 aa) | SRP_receptor_beta_su (21/138 aa) CDS Deleted
ENSMUSE00000277617 Small_GTPase (47/161 aa) | MIRO-like (47/114 aa) | Small_GTPase_ARF/SAR (47/160 aa) | ProtSyn_GTP-bd (47/158 aa) | Gtr1_RagA (47/137 aa) | SRP_receptor_beta_su (47/138 aa) CDS CDS + stop codon + UTR
ENSMUSE00000134728 Small_GTPase (30/161 aa) | MIRO-like (17/114 aa) | Small_GTPase_ARF/SAR (30/160 aa) | ProtSyn_GTP-bd (30/158 aa) | Gtr1_RagA (30/137 aa) | SRP_receptor_beta_su (30/138 aa) CDS
ENSMUSE00000277563 Small_GTPase (34/161 aa) | Small_GTPase_ARF/SAR (30/160 aa) | ProtSyn_GTP-bd (32/158 aa) | Gtr1_RagA (10/137 aa) | SRP_receptor_beta_su (10/138 aa) CDS
ENSMUSE00000460061 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000167962 protein_coding No protein product (NMD) view

Predicted translation:

MDPSAALHRRPAGGSLGAVSPALSGGQARRRKQPPRPADFKLQVIIIGSRGVGKTSLMERFTDDTFCEACKSTVGTQQGR
SDLTALPQPITEVPRGSYSYMTSPRRRRSMTCQSG*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000913147 Small_GTPase (31/161 aa) | MIRO-like (31/114 aa) | Small_GTPase_ARF/SAR (34/160 aa) | ProtSyn_GTP-bd (30/158 aa) | Gtr1_RagA (31/137 aa) | SRP_receptor_beta_su (32/138 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001066698 Small_GTPase (21/161 aa) | MIRO-like (21/114 aa) | Small_GTPase_ARF/SAR (21/160 aa) | ProtSyn_GTP-bd (21/158 aa) | Gtr1_RagA (21/137 aa) | SRP_receptor_beta_su (21/138 aa) CDS Deleted
ENSMUSE00000277617 Small_GTPase (47/161 aa) | MIRO-like (47/114 aa) | Small_GTPase_ARF/SAR (47/160 aa) | ProtSyn_GTP-bd (47/158 aa) | Gtr1_RagA (47/137 aa) | SRP_receptor_beta_su (47/138 aa) CDS CDS + stop codon + UTR
ENSMUSE00000134728 Small_GTPase (30/161 aa) | MIRO-like (17/114 aa) | Small_GTPase_ARF/SAR (30/160 aa) | ProtSyn_GTP-bd (30/158 aa) | Gtr1_RagA (30/137 aa) | SRP_receptor_beta_su (30/138 aa) CDS
ENSMUSE00000277563 Small_GTPase (34/161 aa) | Small_GTPase_ARF/SAR (30/160 aa) | ProtSyn_GTP-bd (32/158 aa) | Gtr1_RagA (10/137 aa) | SRP_receptor_beta_su (10/138 aa) CDS
ENSMUSE00000460061 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000146279 retained_intron No analysis available: incomplete transcript
ENSMUST00000155026 retained_intron Target region does not overlap transcript
ENSMUST00000135620 processed_transcript No analysis available: incomplete transcript
show/hide more transcripts

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available