IKMC Project Report - KOMP-CSD (ID: 33290)

3110002H16Rik MGI:1916528 ENSMUSG00000024410 OTTMUSG00000028184
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 9 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000025276 protein_coding No protein product (NMD) view

Predicted translation:

MGGEDYYLELCERPVQFEKANPVNCVFFDEANKQVFAVRSGGATGVVVKGPDDRNPISFRTFVILFQITLNWSTHKSARP
RMPTYSDSAGPALLRSSS*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000969443 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000985617 CDS CDS
ENSMUSE00001067817 CDS Deleted
ENSMUSE00000972352 CDS CDS
ENSMUSE00001061878 CDS CDS + stop codon + UTR
ENSMUSE00001058527 CDS
ENSMUSE00001035733 CDS
ENSMUSE00001016526 CDS
ENSMUSE00000996766 CDS
ENSMUSE00001030062 CDS
ENSMUSE00000287197 CDS
ENSMUSE00000287175 CDS
ENSMUSE00001012688 CDS
ENSMUSE00001013638 CDS
ENSMUSE00001085257 CDS
ENSMUSE00000969864 Mic1 (26/166 aa) CDS
ENSMUSE00001024865 Mic1 (35/166 aa) CDS
ENSMUSE00001021547 Mic1 (23/166 aa) CDS
ENSMUSE00001021055 Mic1 (76/166 aa) CDS
ENSMUSE00000813369 Mic1 (7/166 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000134046 protein_coding Target region does not overlap transcript
ENSMUST00000138866 nonsense_mediated_decay Target region does not overlap transcript
ENSMUST00000139151 retained_intron Target region does not overlap transcript
ENSMUST00000127123 retained_intron No analysis available: incomplete transcript
ENSMUST00000126523 retained_intron No analysis available: incomplete transcript
ENSMUST00000153233 retained_intron Target region does not overlap transcript
ENSMUST00000155431 retained_intron No analysis available: incomplete transcript
ENSMUST00000134756 retained_intron Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available