IKMC Project Report - (ID: 33753)

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Pre-pipeline Designs Vectors ES Cells Mice

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 2 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000033331 protein_coding No protein product (NMD) view

Predicted translation:

MFYSGLLTEGGRKETDMREAASLRQQRRMKQAVQFIHKDSADLLPLDGLKKLGSSKDTTKGPCQISQA*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000589282 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000205608 Peptidase_aspartic_euk-pred (7/104 aa) CDS Deleted
ENSMUSE00000205606 Peptidase_aspartic_euk-pred (28/104 aa) CDS Deleted
ENSMUSE00000205613 Peptidase_aspartic_euk-pred (48/104 aa) CDS CDS + stop codon + UTR
ENSMUSE00000205612 Peptidase_aspartic_euk-pred (18/104 aa) CDS
ENSMUSE00000205610 Peptidase_aspartic_euk-pred (5/104 aa) CDS
ENSMUSE00000469701 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000041460 protein_coding No protein product (NMD) view

Predicted translation:

MAQLSVIPGSAAAWTGLLTEGGRKETDMREAASLRQQRRMKQAVQFIHKDSADLLPLDGLKKLGSSKDTTKGPCQISQA*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000528050 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000333739 CDS CDS
ENSMUSE00000205608 Peptidase_aspartic_euk-pred (7/104 aa) CDS Deleted
ENSMUSE00000205606 Peptidase_aspartic_euk-pred (28/104 aa) CDS Deleted
ENSMUSE00000205613 Peptidase_aspartic_euk-pred (48/104 aa) CDS CDS + stop codon + UTR
ENSMUSE00000205612 Peptidase_aspartic_euk-pred (18/104 aa) CDS
ENSMUSE00000205610 Peptidase_aspartic_euk-pred (5/104 aa) CDS
ENSMUSE00000841158 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available