IKMC Project Report - KOMP-CSD (ID: 36051)

Mettl21e MGI:2685837 ENSMUSG00000046828 OTTMUSG00000026594
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - Targeting Confirmed

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). This mutation is of type 'Deletion' (more information on IKMC alleles can be found here). The table below shows the predicted structure of the gene transcripts. Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000054801 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MDLTVTHITHKETYK

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000786755 UTR UTR
ENSMUSE00000984601 UTR + start codon + CDS
ENSMUSE00000346955 Nicotinamide_N-MeTfrase-like (17/163 aa) CDS Deleted
ENSMUSE00000438058 Nicotinamide_N-MeTfrase-like (40/163 aa) | Ribosomal-L11_MeTrfase_PrmA (26/86 aa) CDS Deleted
ENSMUSE00000400582 Nicotinamide_N-MeTfrase-like (107/163 aa) | Ribosomal-L11_MeTrfase_PrmA (61/86 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000150062 retained_intron No analysis available: incomplete transcript
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ES Cell Clones Without Conditional Potential (Deletions)

Note: Mutations of type "Deletion" are correctly targeted clones that have had the target exon removed. These mutations cannot be converted into conditional alleles.

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool - LRPCR Genotyping Primers
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order DEPD00567_2_D06 PG00107_Y_4_C05 Mettl21etm1(KOMP)Mbp Deletion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order DEPD00567_2_A07 PG00107_Y_4_C05 Mettl21etm1(KOMP)Mbp Deletion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity pass
Distribution Centre
Karyotype - Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order DEPD00567_2_A06 PG00107_Y_4_C05 Mettl21etm1(KOMP)Mbp Deletion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity pass
Distribution Centre
Karyotype - Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order DEPD00567_2_E05 PG00107_Y_4_C05 Mettl21etm1(KOMP)Mbp Deletion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
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Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 99226 Deletion (Promotor Driven Cassette) PG00107_Y_4_C05 L1L2_Bact_P L4L3_pD223_DTA_spec view
order 99226 Deletion (Promotor Driven Cassette) PG00107_Z_4_C05 L1L2_Bact_P L4L3_pD223_DTA_spec view
order 99226 Deletion (Promotor Driven Cassette) PG00107_Z_3_C05 L1L2_Bact_P L4L3_pD223_DTA_spec view
order 99226 Deletion (Promotor Driven Cassette) PG00107_Z_2_C05 L1L2_Bact_P L4L3_pD223_DTA_spec view
order 99226 Deletion (Promotor Driven Cassette) PG00107_Z_4_B05 L1L2_Bact_P L4L3_pD223_DTA_spec view
order 99226 Deletion (Promotor Driven Cassette) PG00107_Z_1_C05 L1L2_Bact_P L4L3_pD223_DTA_spec view
order 99226 Deletion (Promotor Driven Cassette) DPGS00107_A_C05 L1L2_Bact_P L4L3_pD223_DTA_spec view
order 99226 Deletion (Promotor Driven Cassette) PG00107_Z_3_B05 L1L2_Bact_P L4L3_pD223_DTA_spec view
order 99226 Deletion (Promotor Driven Cassette) PG00107_Z_1_B05 L1L2_Bact_P L4L3_pD223_DTA_spec view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
99226 Deletion PCS00107_A_C05