IKMC Project Report - KOMP-CSD (ID: 36563)

Cspg4 MGI:2153093 ENSMUSG00000032911
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000035661 protein_coding No protein product (NMD) view

Predicted translation:

MLLGPGHPLSAPALALALTLALLVRSTAPGQTCPGTEGAKAADASRHRAE*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000263224 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000263196 Laminin_G (29/124 aa) | Laminin_G (29/127 aa) CDS Deleted
ENSMUSE00000263166 Laminin_G (95/124 aa) | Laminin_G (133/133 aa) | Laminin_G (98/127 aa) | Laminin_G (133/133 aa) CDS CDS + stop codon + UTR
ENSMUSE00000263134 CDS
ENSMUSE00000263113 CDS
ENSMUSE00000263093 CDS
ENSMUSE00000263068 CDS
ENSMUSE00000263048 CDS
ENSMUSE00000263029 CDS
ENSMUSE00000263006 CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available