IKMC Project Report - KOMP-CSD (ID: 36847)

L1cam MGI:96721 ENSMUSG00000031391 OTTMUSG00000016517
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 13 wild type transcripts, of which 6 are protein-coding. Following removal of the floxed region, 6 transcripts are predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000102871 protein_coding No protein product (NMD) view

Predicted translation:

MVVMLRYVWPLLLCSPCLLIQIPDESLHPPSSGCTPVTQCQQTVLSTKTTTRPCNYSMWAKRTMASIPALLRTRWAVPGM
PTMLLWKLPHIGCRSPRAICMVQERLPA*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000662869 UTR UTR
ENSMUSE00001018230 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001079468 CDS Deleted
ENSMUSE00001048707 Ig_I-set (27/85 aa) CDS Deleted
ENSMUSE00000983203 Ig_I-set (59/85 aa) CDS Deleted
ENSMUSE00001006009 CDS Deleted
ENSMUSE00001033223 CDS Deleted
ENSMUSE00001080226 Ig_I-set (21/82 aa) | Immunoglobulin (12/57 aa) CDS Deleted
ENSMUSE00000980123 Ig_I-set (62/82 aa) | Immunoglobulin (46/57 aa) CDS CDS
ENSMUSE00000209065 Ig_I-set (39/76 aa) CDS CDS + stop codon + UTR
ENSMUSE00000209084 Ig_I-set (38/76 aa) CDS
ENSMUSE00000209083 Ig_I-set (27/82 aa) | Ig_V-set (21/66 aa) CDS
ENSMUSE00000209068 Ig_I-set (56/82 aa) | Ig_V-set (46/66 aa) CDS
ENSMUSE00000209061 Ig_I-set (49/90 aa) CDS
ENSMUSE00001003164 Ig_I-set (42/90 aa) CDS
ENSMUSE00000988005 Fibronectin_type3 (20/75 aa) CDS
ENSMUSE00000982881 Fibronectin_type3 (56/75 aa) CDS
ENSMUSE00001062471 Fibronectin_type3 (18/82 aa) CDS
ENSMUSE00000997492 Fibronectin_type3 (64/82 aa) CDS
ENSMUSE00001047637 Fibronectin_type3 (24/83 aa) CDS
ENSMUSE00001023500 Fibronectin_type3 (59/83 aa) CDS
ENSMUSE00001031783 Fibronectin_type3 (37/83 aa) CDS
ENSMUSE00001068647 Fibronectin_type3 (47/83 aa) CDS
ENSMUSE00000977520 CDS
ENSMUSE00000999930 CDS
ENSMUSE00001019623 CDS
ENSMUSE00001076757 CDS
ENSMUSE00001003605 CDS
ENSMUSE00000799694 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000114430 protein_coding No analysis available: incomplete transcript
ENSMUST00000066576 protein_coding No analysis available: incomplete transcript
ENSMUST00000146790 protein_coding No analysis available: incomplete transcript
ENSMUST00000148250 protein_coding Target region does not overlap transcript
ENSMUST00000124560 protein_coding Target region does not overlap transcript
ENSMUST00000129612 retained_intron No analysis available: incomplete transcript
ENSMUST00000130110 retained_intron Target region does not overlap transcript
ENSMUST00000145805 retained_intron Target region does not overlap transcript
ENSMUST00000144478 retained_intron Target region does not overlap transcript
ENSMUST00000155246 retained_intron Target region does not overlap transcript
ENSMUST00000135104 retained_intron Target region does not overlap transcript
ENSMUST00000141221 processed_transcript No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available