IKMC Project Report - EUCOMM (ID: 36979)

2610020H08Rik MGI:1919402 ENSMUSG00000030924 OTTMUSG00000025657
Pre-pipeline Designs Vectors ES Cells Mice
Vector Construction in Progress

Mice no data available

Mutagenesis Predictions

This gene has 9 wild type transcripts, of which 3 are protein-coding. Following removal of the floxed region, 3 transcripts are predicted to produce a truncated protein product of which 2 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000033218 protein_coding No protein product (NMD) view

Predicted translation: 

MEQEKEEANSRKRKEAPNSSASATERDGPPSHIQDREPQAKKARLSTILFTDNCEVTHRQLCELLKYAVLGKSAVPKPSW
CQLSHQKQLNNVVVFILKGMSQLHFYRFYLEFRFLRKTFRHKFSLPSPTSSFLFDIIGLQKKKSARSFRRTVEGPLISAT
LRSSIDLQNDPIIQKYGYKNVGLTKCLLTKEEMKTFHFPLPVPYIHGERTNTHLTGY*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000835565 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000343223 CDS CDS
ENSMUSE00000384497 CDS CDS
ENSMUSE00000337866 CDS CDS
ENSMUSE00000991695 CDS CDS
ENSMUSE00001039106 Exonuclease_RNaseT/DNA_pol3 (6/93 aa) CDS Deleted
ENSMUSE00001092310 Exonuclease_RNaseT/DNA_pol3 (39/93 aa) CDS CDS + stop codon + UTR
ENSMUSE00001089910 Exonuclease_RNaseT/DNA_pol3 (47/93 aa) CDS
ENSMUSE00001047056 Exonuclease_RNaseT/DNA_pol3 (2/93 aa) CDS
ENSMUSE00001063538 CDS
ENSMUSE00000982586 CDS
ENSMUSE00001013030 CDS
ENSMUSE00001070072 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000106520 protein_coding No protein product (NMD) view

Predicted translation: 

MEQEKEEANSRKRKEAPNSSASATERDGPPSHIQDREPQAKKARLSTILFTDNCEVTHRQLCELLKYAVLGKSAVPKPSW
CQLSHQKQLNNVVVFILKGMSQLHFYRFYLEFRFLRKTFRHKFSLPSPTSSFLFDIIGLQKKKSARSFRRTVEGPLISAT
LRSSIDLQNDPIIQKYGYKNVGLTKCLLTKEEMKTFHFPLPVPYIHGERTNTHLTGY*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000670349 UTR UTR
ENSMUSE00000670348 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000343223 CDS CDS
ENSMUSE00000384497 CDS CDS
ENSMUSE00000337866 CDS CDS
ENSMUSE00000991695 CDS CDS
ENSMUSE00001039106 Exonuclease_RNaseT/DNA_pol3 (6/148 aa) CDS Deleted
ENSMUSE00001092310 Exonuclease_RNaseT/DNA_pol3 (39/148 aa) CDS CDS + stop codon + UTR
ENSMUSE00001089910 Exonuclease_RNaseT/DNA_pol3 (47/148 aa) CDS
ENSMUSE00000976549 Exonuclease_RNaseT/DNA_pol3 (32/148 aa) CDS
ENSMUSE00001045514 Exonuclease_RNaseT/DNA_pol3 (26/148 aa) CDS
ENSMUSE00000670347 CDS
ENSMUSE00001063538 CDS
ENSMUSE00000982586 CDS
ENSMUSE00001013030 RRM_dom (32/64 aa) CDS
ENSMUSE00001055349 RRM_dom (31/64 aa) CDS
ENSMUSE00000976306 RRM_dom (1/64 aa) CDS
ENSMUSE00000588605 CDS
ENSMUSE00000588604 CDS
ENSMUSE00001016260 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000084644 protein_coding Residual C-terminal product view

Predicted translation: 

MGKELTRISLVTEGGYCLIDELVKPDLKILDYLTSFTGITKEILNPVTTKLKDVQKLLRELLPPDAVLVGHCLDLDLRVL
KMIHPYVIDTSLLYAGKQGRRFKLTFLARVILGKDIQCPNKLGRDGIEDARAALELLQYFLKYGPKKIAEFNLEALAANQ
EQGDKEEAAHMSVLKCLESMGQKLLFLTQDINELSSYRNCQTIKCSSNKKVLEQARVEVPLFPFNIVEFSFQPFPPLFAE
EMKNSMKTKWTQMSTVYAGPFSKDCNVGALKKVFSSLGPVHSITLVLETYRPYFSIQYEVLEAAQLALETMNGSLLEGSC
IRVRRLVTELTLECDTLVRELEQDSENQGTIYVAGIGETFKEHLLEQSNLFPDLEAVILPKEVKSRKQKNYCFLMLLGRT
LA*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00001014080 UTR UTR
ENSMUSE00000975519 UTR + start codon + CDS
ENSMUSE00001039106 Exonuclease_RNaseT/DNA_pol3 (6/148 aa) CDS Deleted
ENSMUSE00001092310 Exonuclease_RNaseT/DNA_pol3 (39/148 aa) CDS UTR + start codon + CDS
ENSMUSE00001089910 Exonuclease_RNaseT/DNA_pol3 (47/148 aa) CDS CDS
ENSMUSE00000976549 Exonuclease_RNaseT/DNA_pol3 (32/148 aa) CDS CDS
ENSMUSE00001045514 Exonuclease_RNaseT/DNA_pol3 (26/148 aa) CDS CDS
ENSMUSE00001047056 CDS CDS
ENSMUSE00001063538 CDS CDS
ENSMUSE00000982586 CDS CDS
ENSMUSE00001013030 RRM_dom (32/64 aa) CDS CDS
ENSMUSE00001055349 RRM_dom (31/64 aa) CDS CDS
ENSMUSE00000976306 RRM_dom (1/64 aa) CDS CDS
ENSMUSE00001028832 CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000133758 nonsense_mediated_decay No protein product (NMD) view

Predicted translation: 

MEQEKEEANSRKRKEAPNSSASATERDGPPSHIQDREPQAKDL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00001068482 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000991663 CDS + stop codon + UTR CDS + stop codon + UTR
ENSMUSE00001006227 UTR Deleted
ENSMUSE00001092515 UTR UTR
ENSMUSE00001052973 UTR UTR
ENSMUSE00001083215 UTR UTR
ENSMUSE00000985694 UTR UTR
ENSMUSE00001078989 UTR UTR
ENSMUSE00000988318 UTR UTR
ENSMUSE00000971728 UTR UTR
ENSMUSE00001056121 UTR UTR
ENSMUSE00000973968 UTR UTR
ENSMUSE00001001715 UTR UTR
ENSMUSE00001060054 UTR UTR
Legend: in frame deleted frameshifted
ENSMUST00000137015 retained_intron No analysis available: incomplete transcript
ENSMUST00000155938 retained_intron Target region does not overlap transcript
ENSMUST00000149132 processed_transcript Target region does not overlap transcript
ENSMUST00000151248 retained_intron Target region does not overlap transcript
ENSMUST00000138689 processed_transcript Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available