IKMC Project Report - EUCOMM (ID: 38195)

Wbscr22 MGI:1913388 ENSMUSG00000005378 OTTMUSG00000025995
Pre-pipeline Designs Vectors ES Cells Mice
Vector Unsuccessful - Project Terminated

Mice no data available

Mutagenesis Predictions

This gene has 13 wild type transcripts, of which 6 are protein-coding. Following removal of the floxed region, 6 transcripts are predicted to produce a truncated protein product of which 2 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000085984 protein_coding No protein product (NMD) view

Predicted translation:

MASRSRRPEHSGPPELFYDQNEARKYVRNSRMIDIQTKMTERALELLCLPEGQPSYLLDIGCGSGLSGDYISEEGHYWVG
IDISPAMLDAALDRDTEGDLLLGDMGQGVPFRPGSFDGCIRSVGPELSCSCTLRTRSSWS*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000495311 UTR UTR
ENSMUSE00000687052 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001007125 CDS CDS
ENSMUSE00001070049 Methyltransf_11 (3/74 aa) CDS CDS
ENSMUSE00001047929 Methyltransf_11 (29/74 aa) CDS CDS
ENSMUSE00001092306 Methyltransf_11 (33/74 aa) CDS CDS
ENSMUSE00001084291 Methyltransf_11 (12/74 aa) CDS Deleted
ENSMUSE00000975637 CDS CDS
ENSMUSE00001080479 CDS CDS + stop codon + UTR
ENSMUSE00001001072 Unchr_MeTrfase_Williams-Beuren (10/77 aa) CDS
ENSMUSE00001031206 Unchr_MeTrfase_Williams-Beuren (20/77 aa) CDS
ENSMUSE00000964655 Unchr_MeTrfase_Williams-Beuren (31/77 aa) CDS
ENSMUSE00000536002 Unchr_MeTrfase_Williams-Beuren (18/77 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000071677 protein_coding No protein product (NMD) view

Predicted translation:

MVLPKTSSRMIDIQTKMTERALELLCLPEGQPSYLLDIGCGSGLSGDYISEEGHYWVGIDISPAMLDAALDRDTEGDLLL
GDMGQGVPFRPGSFDGCIRSVGPELSCSCTLRTRSSWS*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000687083 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001070049 Methyltransf_11 (3/74 aa) CDS CDS
ENSMUSE00001047929 Methyltransf_11 (29/74 aa) CDS CDS
ENSMUSE00001092306 Methyltransf_11 (33/74 aa) CDS CDS
ENSMUSE00001084291 Methyltransf_11 (12/74 aa) CDS Deleted
ENSMUSE00000975637 CDS CDS
ENSMUSE00001080479 CDS CDS + stop codon + UTR
ENSMUSE00001001072 Unchr_MeTrfase_Williams-Beuren (10/77 aa) CDS
ENSMUSE00001031206 Unchr_MeTrfase_Williams-Beuren (20/77 aa) CDS
ENSMUSE00000964655 Unchr_MeTrfase_Williams-Beuren (31/77 aa) CDS
ENSMUSE00000649050 Unchr_MeTrfase_Williams-Beuren (18/77 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000111205 protein_coding No analysis available: incomplete transcript
ENSMUST00000148549 protein_coding No analysis available: incomplete transcript
ENSMUST00000141309 protein_coding Target region does not overlap transcript
ENSMUST00000129691 protein_coding Target region does not overlap transcript
ENSMUST00000144891 retained_intron No analysis available: incomplete transcript
ENSMUST00000147756 retained_intron Target region does not overlap transcript
ENSMUST00000149108 retained_intron No analysis available: incomplete transcript
ENSMUST00000141765 processed_transcript No analysis available: incomplete transcript
ENSMUST00000134180 retained_intron Target region does not overlap transcript
ENSMUST00000151551 retained_intron Target region does not overlap transcript
ENSMUST00000129013 processed_transcript Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available