IKMC Project Report - EUCOMM (ID: 39975)

Aass MGI:1353573 ENSMUSG00000029695 OTTMUSG00000023540
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 4 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000031707 protein_coding No protein product (NMD) view

Predicted translation:

MLRAQRPRLARLRACLSRGLHHKPVMALRREDVNAWERRAPLAPKHIKGITKLGYKVLIQPSNRRAIHDKEYVRAGGILQ
EDITEACLILGVKRPPEEKLMSKKTYAFFSHTIKAQEANMNLLDEVLKQE*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000619468 UTR UTR
ENSMUSE00000419492 AlaDH/PNT_N (42/130 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000419480 AlaDH/PNT_N (59/130 aa) CDS CDS
ENSMUSE00000325827 AlaDH/PNT_N (29/130 aa) CDS Deleted
ENSMUSE00000325823 AlaDH/PNT_N (1/130 aa) CDS CDS + stop codon + UTR
ENSMUSE00000325818 AlaDH/PNT_NAD(H)-bd (32/203 aa) CDS
ENSMUSE00000325812 AlaDH/PNT_NAD(H)-bd (27/203 aa) CDS
ENSMUSE00001088883 AlaDH/PNT_NAD(H)-bd (43/203 aa) CDS
ENSMUSE00000564853 AlaDH/PNT_NAD(H)-bd (50/203 aa) CDS
ENSMUSE00000419453 AlaDH/PNT_NAD(H)-bd (42/203 aa) CDS
ENSMUSE00000419447 AlaDH/PNT_NAD(H)-bd (12/203 aa) CDS
ENSMUSE00001034739 CDS
ENSMUSE00001029734 CDS
ENSMUSE00001038869 Saccharopine_DH/HSpermid_syn (27/436 aa) CDS
ENSMUSE00000997414 Saccharopine_DH/HSpermid_syn (43/436 aa) CDS
ENSMUSE00000998164 Saccharopine_DH/HSpermid_syn (38/436 aa) CDS
ENSMUSE00000419410 Saccharopine_DH/HSpermid_syn (37/436 aa) CDS
ENSMUSE00000419403 Saccharopine_DH/HSpermid_syn (47/436 aa) CDS
ENSMUSE00000325775 Saccharopine_DH/HSpermid_syn (56/436 aa) CDS
ENSMUSE00000419389 Saccharopine_DH/HSpermid_syn (32/436 aa) CDS
ENSMUSE00000564828 Saccharopine_DH/HSpermid_syn (39/436 aa) CDS
ENSMUSE00000191640 Saccharopine_DH/HSpermid_syn (31/436 aa) CDS
ENSMUSE00000191638 Saccharopine_DH/HSpermid_syn (60/436 aa) CDS
ENSMUSE00000501743 Saccharopine_DH/HSpermid_syn (32/436 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000149864 protein_coding No analysis available: incomplete transcript
ENSMUST00000138063 processed_transcript No analysis available: incomplete transcript
ENSMUST00000152280 processed_transcript No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available