IKMC Project Report - KOMP-CSD (ID: 45194)

Actr1a MGI:1858964 ENSMUSG00000025228
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000040270 protein_coding No protein product (NMD) view

Predicted translation:

MESYDVIANQPVVIDNGSGVIKAGFAGDQIPKYCFPNYVGRPKHVRVMAGALEGDIFIGPKAEEHRGLLSIRYPMEHGIV
KDWNDMERIWQYVYSKDQLQTFSEELCHGQNHRRGVGFWGWCHPCSPDL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000422129 Actin-like (7/368 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000149082 Actin-like (22/368 aa) CDS CDS
ENSMUSE00000149085 Actin-like (26/368 aa) CDS CDS
ENSMUSE00000542425 Actin-like (42/368 aa) CDS CDS
ENSMUSE00000519925 Actin-like (42/368 aa) CDS Deleted
ENSMUSE00000149080 Actin-like (73/368 aa) CDS CDS + stop codon + UTR
ENSMUSE00000149084 Actin-like (31/368 aa) CDS
ENSMUSE00000149087 Actin-like (59/368 aa) CDS
ENSMUSE00000542423 Actin-like (21/368 aa) CDS
ENSMUSE00000361593 Actin-like (14/368 aa) CDS
ENSMUSE00000422115 Actin-like (34/368 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available