IKMC Project Report - KOMP-CSD (ID: 48746)

Cpn1 MGI:2135874 ENSMUSG00000025196 OTTMUSG00000026385
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000026210 protein_coding No protein product (NMD) view

Predicted translation:

MPDLPSAFLPLLLLSKFVTPVTFRHHRYDDLVRTLYKVHNQCPDITRLYNIGRSVKGRYLYVLEFSDYPGIHEPWPKHVW
VSGW*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000389981 Peptidase_M14 (42/297 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000148750 Peptidase_M14 (66/297 aa) CDS Deleted
ENSMUSE00000432384 Peptidase_M14 (52/297 aa) CDS CDS + stop codon + UTR
ENSMUSE00000432378 Peptidase_M14 (61/297 aa) CDS
ENSMUSE00000432371 Peptidase_M14 (38/297 aa) CDS
ENSMUSE00001056734 Peptidase_M14 (40/297 aa) CDS
ENSMUSE00000271230 CDS
ENSMUSE00000271201 CDS
ENSMUSE00000404776 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000131882 retained_intron Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available